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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combined nitric oxide-releasing poly(vinyl alcohol) film/F127 hydrogel for accelerating wound healing

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Schanuel, Fernanda Seabra [1] ; Raggio Santos, Karen Slis [2] ; Monte-Alto-Costa, Andrea [1] ; de Oliveira, Marcelo G. [2]
Total Authors: 4
[1] Univ Estado Rio De Janeiro, UERJ, Dept Histol & Embryol, BR-20950003 Rio De Janeiro - Brazil
[2] Univ Estadual Campinas, Univ Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 130, p. 182-191, JUN 1 2015.
Web of Science Citations: 31

Nitric oxide (NO) releasing biomaterials represent a potential strategy for use as active wound dressings capable of accelerating wound healing. Topical NO-releasing poly(vinyl alcohol) (PVA) films and Pluronic F127 hydrogels (F127) have already exhibited effective skin vasodilation and wound healing actions. In this study, we functionalized PVA films with SNO groups via esterification with a mixture of mercaptosucinic acid (MSA) and thiolactic acid (TLA) followed by S-nitrosation of the -SH moieties. These films were combined with an underlying layer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), i.e., PEO-PPO-PEO (Pluronic F127) hydrogel and used for the topical treatment of skin lesions in an animal model. The mixed esterification of PVA with MSA and TLA led to chemically crosslinked PVA-SNO films with a high swelling capacity capable of spontaneously releasing NO. Real time NO-release measurements revealed that the hydrogel layer reduces the initial NO burst from the PVA-SNO films. We demonstrate that the combination of PVA-SNO films with F127 hydrogel accelerates wound contraction, decreases wound gap and cellular density and accelerates the inflammatory phase of the lesion. These results were reflected in an increase in myofibroblastic differentiation and collagen type III expression in the cicatricial tissue. Therefore, PVA-SNO films combined with F127 hydrogel may represent a new approach for active wound dressings capable of accelerating wound healing. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/57560-0 - Effects of green tea (Camellia sinensis), cocoa and nitric oxide donor on diabetic nephropathy and retinopathy: contribution of the reduction of oxidative stress and inflammation and elevation of nitric oxide
Grantee:Jose Butori Lopes de Faria
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/21936-4 - Poly(vinyl alcohol) films functionalized with SNO groups for the topical treatment of lesions
Grantee:Karen Slis Raggio Santos
Support type: Scholarships in Brazil - Scientific Initiation