| Grant number: | 13/21936-4 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | April 01, 2014 |
| Effective date (End): | April 30, 2015 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry - Physical-Chemistry |
| Principal Investigator: | Marcelo Ganzarolli de Oliveira |
| Grantee: | Karen Slis Raggio Santos |
| Home Institution: | Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
Abstract The topical application of nitric oxide (NO) releasing biomaterials has shown promising results for accelerating wound healing, especially in chronic ulcers. NO-releasing hydrogels have already shown effective action in the acceleration of wound healing in both inflammatory and proliferative phases. In addition, several works describe beneficial actions resulting from simply coating lesions with hydrophilic polymeric films. The objectives of this project are the preparation and characterization of hydrophylic poly(vinyl alcohol) (PVA) films functionalized with NO donor SNO groups and their topical applications for the promotion of dermal vasodilation in the health skin and for the wound healing in animal model. PVA will be functionalized with SNO groups through its esterification reaction with sulfhydrylated carboxylic acids, followed by S-nitrosation of the SH groups. The films, prepared by casting, will be characterized relative to their NO charge and the kinetics of NO release in physiological medium, using spectrophotometric and chemiluminescence techniques. The dermal vasodilation action of the films will be evaluated by laser Doppler flowmetry and the films will also be used in a in a wound healing animal model study using Swiss mice, in collaboration with Prof. Andrea Monte Alto Costa, from the Department of Histology and Embryology of the UFRJ. The in vitro kinetic data of NO release will be correlated with the in vivo vasodilation and wound healing results, in order to select the formulations with potential for clinical use. (AU) | |