Nitric oxide releasing collagen membranes for bone regeneration

• Grant number: 17/04615-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2017
• End date: January 01, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Marcelo Ganzarolli de Oliveira
• Grantee: Giovanna Jardim Vieira Pereira dos Santos
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release, AP.TEM
• Associated scholarship(s): 19/00913-2 - Poly(vinyl alcohol) effect on the micellar packing and photochemical nitric oxide release from S-nitrosoglutathione in Pluronic F-127 solutions, BE.EP.IC

Abstract

Bioabsorbable biomaterials have been widely used in the treatments of bone defects. Collagen has good biocompatibility, weak antigenic activity, and has already been commercially used as membranes with hemostatic action and for wound dressing. Nitric oxide (NO) has osteogenic activity characterized by the induction of increased osteoblastic activity and reduced osteoclastic activity. The main aim of this project is the preparation of type I collagen membranes functionalized with S-nitrosothiol groups and charged with S-nitrosoglutathione (GSNO), for the localized NO release. Membranes functionalization will be achieved through the grafting of cysteine, N-acethylcysteine and glutathione, followed by nitrosation with nitrous acid (HONO). GSNO incorporation will be achieved through sorption from solution. The membranes will be analyzed by chemiluminescence regarding their charges and real time NO release kinetics. The charges of SNO groups will be adjusted to stimulate osteoblasts proliferation in cultured cells aiming therapeutic action in bone regeneration. The biodegradability of the membranes will be characterized using enzymatic degradation assay for collagenase. The results obtained are expected to contribute to the development of new NO releasing absorbable scafolds, according to one of the sub-projects of the Thematic Project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POVOA, VALERIA C. O.; DOS SANTOS, GIOVANNA J. V. P.; PICHETH, GUILHERME F.; JARA, CARLOS P.; DA SILVA, LAURA C. E.; DE ARAUJO, ELIANA P.; DE OLIVEIRA, MARCELO G.. Wound healing action of nitric oxide-releasing self-expandable collagen sponge. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 14, n. 6, MAY 2020. (13/07607-8, 18/14142-5, 17/04615-0, 16/02414-5, 17/19253-7)

PICHETH, GUILHERME F.; MARINI, THATIANE C.; TALADRIZ-BLANCO, PATRICIA; SHIMAMOTO, GUSTAVO G.; DOS SANTOS, GIOVANNA J. V. P.; MENEAU, FLORIAN; DE OLIVEIRA, MARCELO G.. Influence of Pluronic F127 microenvironments on the photochemical nitric oxide release from S-nitrosoglutathione. Journal of Colloid and Interface Science, v. 544, p. 217-229, MAY 15 2019. (16/02414-5, 17/19253-7, 14/03266-4, 17/04615-0, 13/07173-8)