Influence of Pluronic F127 microenvironments on the photochemical nitric oxide release from S-nitrosoglutathione

Author(s)

Picheth, Guilherme F. ^[1] ; Marini, Thatiane C. ^[1] ; Taladriz-Blanco, Patricia ^{[1, 2]} ; Shimamoto, Gustavo G. ^[1] ; dos Santos, Giovanna J. V. P. ^[1] ; Meneau, Florian ^[3] ; de Oliveira, Marcelo G. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, UNICAMP, CP 6154, BR-13083970 Campinas, SP - Brazil

^[2] Adolphe Merkle Inst, Chemin Verdiers 4, CH-1700 Fribourg - Switzerland

^[3] Brasilian Synchroton Light Lab, LNLS, CP 6192, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Colloid and Interface Science; v. 544, p. 217-229, MAY 15 2019.
• Web of Science Citations: 1

Abstract

Poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) (F127) hydrogels have been used to deliver nitric oxide (NO) topically in biomedical applications. Here, the effect of F127 microenvironments on the photochemical NO release from S-nitrosoglutathione (GSNO) was investigated in F127 solutions 7.6 wt% 15 wt% and 22.5 wt% at 15 degrees C and 37 degrees C. Small-angle X-ray Scattering (SAXS) and Differential Scanning Calorimetry (DSC) measurements, along with proton Nuclear Magnetic Resonance (H-1 NMR) spectral shifts and T-2 relaxation data at six different concentration-temperature conditions, allowed identifying F127 microphases characterized by: a sol phase of unimers; micelles in non-defined periodic order, and a gel phase of cubic packed micelles. Kinetic measurements showed that GSNO photodecompositon proceeds faster in micellized F127 where GSNO is segregated to the intermicellar microenvironment. Real time kinetic monitoring of NO release and T-2 relaxation profiles showed that NO is preferentially partitioned into the hydrophobic PPO cores of the F127 micelles, with the consequent decrease in its rate of release to the gas phase. These results show that F127 microphases affect both the kinetics of GSNO photodecomposition and the rate of NO escape and can be used to modulate the photochemical NO delivery from F127/GSNO solutions. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 17/04615-0 - Nitric oxide releasing collagen membranes for bone regeneration
• Grantee: Giovanna Jardim Vieira Pereira dos Santos
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/19253-7 - Pluronic F127 thermosensitive hydrogels functionalized with terminal groups releasing nitric oxide
• Grantee: Guilherme Fadel Picheth
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 14/03266-4 - PVA-F-127 thermosensitive nanocarriers for the photochemical nitric oxide release
• Grantee: Thatiane Cristine Camargo Marini
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release
• Grantee: Marcelo Ganzarolli de Oliveira
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 13/07173-8 - Nanoparticles and hydrogels for the photochemical delivery of nitric oxide
• Grantee: Patricia Taladriz Blanco
• Support type: Scholarships in Brazil - Post-Doctorate