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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Low-Level Laser Therapy (LLLT) in Dystrophin-Deficient Muscle Cells: Effects on Regeneration Capacity, Inflammation Response and Oxidative Stress

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Autor(es):
Macedo, Aline Barbosa [1] ; Rapucci Moraes, Luis Henrique [1] ; Mizobuti, Daniela Sayuri [1] ; Fogaca, Aline Reis [1] ; Rapucci Moraes, Fernanda dos Santos [1] ; Hermes, Tulio de Almeida [1] ; Pertille, Adriana [2] ; Minatel, Elaine [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Estrut & Func, Campinas, SP - Brazil
[2] Univ Metodista Piracicaba UNIMEP, Grad Program Sci Human Movement, Piracicaba, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 10, n. 6 JUN 17 2015.
Citações Web of Science: 9
Resumo

The present study evaluated low-level laser therapy (LLLT) effects on some physiological pathways that may lead to muscle damage or regeneration capacity in dystrophin-deficient muscle cells of mdx mice, the experimental model of Duchenne muscular dystrophy (DMD). Primary cultures of mdx skeletal muscle cells were irradiated only one time with laser and analyzed after 24 and 48 hours. The LLLT parameter used was 830 nm wavelengths at 5 J/cm(2) fluence. The following groups were set up: Ctrl (untreated C57BL/10 primary muscle cells), mdx (untreated mdx primary muscle cells), mdx LA 24 (mdx primary muscle cells - LLLT irradiated and analyzed after 24 h), and mdx LA 48 (mdx primary muscle cells - LLLT irradiated and analyzed after 48 h). The mdx LA 24 and mdx LA 48 groups showed significant increase in cell proliferation, higher diameter in muscle cells and decreased MyoD levels compared to the mdx group. The mdx LA 48 group showed significant increase in Myosin Heavy Chain levels compared to the untreated mdx and mdx LA 24 groups. The mdx LA 24 and mdx LA 48 groups showed significant increase in {[}Ca2+]i. The mdx group showed significant increase in H2O2 production and 4-HNE levels compared to the Ctrl group and LLLT treatment reduced this increase. GSH levels and GPx, GR and SOD activities increased in the mdx group. Laser treatment reduced the GSH levels and GR and SOD activities in dystrophic muscle cells. The mdx group showed significant increase in the TNF-alpha and NF-kappa B levels, which in turn was reduced by the LLLT treatment. Together, these results suggest that the laser treatment improved regenerative capacity and decreased inflammatory response and oxidative stress in dystrophic muscle cells, indicating that LLLT could be a helpful alternative therapy to be associated with other treatment for dystrophinopathies. (AU)

Processo FAPESP: 13/17299-9 - Efeito da associação de anti-inflamatório e antioxidante sobre células musculares distróficas
Beneficiário:Aline Reis Fogaça
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 10/01087-4 - Tratamento in vivo e in vitro com a associação de N-acetilcisteína e deferoxamina em camundongos distróficos
Beneficiário:Luis Henrique Rapucci Moraes
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/02474-4 - Tratamento in vivo e in vitro com bloqueador de cálcio e antioxidante em camundongos mdx
Beneficiário:Elaine Minatel
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/50189-1 - Influência do ácido ascórbico no processo de degeneração muscular em camundongos distróficos
Beneficiário:Elaine Minatel
Linha de fomento: Auxílio à Pesquisa - Regular