Effectiveness of different methodologies for the selective enumeration of Lactobacillus acidophilus La5 from yoghurt and Prato cheese

Autor(es)

Gebara, Clarice ^[1] ; Ribeiro, Maria Cecilia E. ^[1] ; Chaves, Karina S. ^[1] ; Gandara, Ana Lourdes N. ^[2] ; Gigante, Mirna L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Technol, BR-13082862 Campinas, SP - Brazil

^[2] Tech High Sch Campinas, BR-13020060 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 64, n. 1, p. 508-513, NOV 2015.
• Citações Web of Science: 2

Resumo

Prior to the selective enumeration of probiotic microorganisms in fermented dairy products, a preliminary evaluation of different culture media and incubation conditions is required to choose a suitable culture medium. The objective of this study was to evaluate the effectiveness of different methodologies for the selective enumeration of Lactobacillus acidophilus in probiotic yoghurt and Prato cheese. For selective enumeration of L acidophilus, four methods were investigated under anaerobic conditions, as follows: MRS agar containing bile solution; MRS agar containing clindamycin solution; and MRS agar containing sorbitol solution, each one incubated at 37 degrees C for 72 h, and MRS agar containing sorbitol incubated at 45 degrees C for 72 h. These methodologies were used for enumeration of both the pure cultures and the microorganisms from probiotic yoghurt and Prato cheese. The results showed that the best selective medium for enumeration of L. acidophilus from yoghurt was MRS agar containing bile incubated at 37 degrees C for 72 h under anaerobic conditions. For enumeration of this probiotics from Prato cheese, MRS agar containing sorbitol incubated at 45 degrees C for 72 h under anaerobic conditions was proven to be the best selective medium. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/54268-9 - Microencapsulação de Lactobacillus acidophilus e aplicação na fabricação de iogurte batido e queijo prato probióticos
• Beneficiário: Mirna Lúcia Gigante
• Modalidade de apoio: Auxílio à Pesquisa - Regular