Identification of compounds from high-fat and extra virgin olive oil-supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry

Autor(es)

dos Santos, Gustavo Aparecido ^[1] ; Ferreira, Monica Siqueira ^[1] ; de Oliveira, Diogo Noin ^[1] ; de Oliveira, Vanessa ^[2] ; Siqueira-Santos, Edilene S. ^[3] ; Correa Cintra, Dennys Esper ^[2] ; Castilho, Roger Frigerio ^[3] ; Velloso, Licio Augusto ^[4] ; Catharino, Rodrigo Ramos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Med Sci, Innovare Biomarkers Lab, BR-13083877 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Sci Appl, Sport Sci Course, BR-13083877 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Sch Med Sci, Expt Neurodegenerat Lab, BR-13083877 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Sch Med Sci, Lab Cell Signaling, BR-13083877 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Mass Spectrometry; v. 50, n. 7, p. 951-958, JUL 2015.
• Citações Web of Science: 4

Resumo

Nonalcoholic steatohepatitis (NASH) is a fatty liver disorder that could be improved with extra virgin olive oil (EVOO) supplementation in diet. We propose the monitoring, in whole mouse liver extracts and in isolated mitochondria, of the absorption of compounds from three different diets: standard (CT), high-fat (HFD) and high-fat supplemented with EVOO (HFSO). Male mice were submitted to one of the following three diets: CT or HFD for 16 weeks or HFD for 8 weeks followed by additional 8 weeks with HFSO. Following this period, liver was extracted for histological evaluation, mitochondria isolation and mass spectrometry analyses. Diets, liver extracts and Percoll-purified mitochondria were analyzed using ESI-MS and the lipidomics approach. Morphological, histological and spectrometric results indicated a decrease in NASH severity with EVOO supplementation in comparison with animals maintained with HFD. Spectrometric data also demonstrated that some compounds presented on the diets are absorbed by the mitochondria. EVOO was shown to be a potential therapeutic alternative in food for NASH. Our results are in accordance with the proposition that the major factor that influences different responses to diets is their composition - and not only calories - especially when it comes to studies on obesity. Copyright (C) 2015 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/00084-2 - Identificação de adulterações e determinação simultânea de nutrientes e contaminantes em alimentos por análise direta em espectrometria de massas e imagem
• Beneficiário: Diogo Noin de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado