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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

CspC regulates the expression of the glyoxylate cycle genes at stationary phase in Caulobacter

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Autor(es):
Santos, Juliana S. [1] ; da Silva, Carolina A. P. T. [1] ; Balhesteros, Heloise [1] ; Lourenco, Rogerio F. [2] ; Marques, Marilis V. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 16, AUG 27 2015.
Citações Web of Science: 4
Resumo

Background: The Cold Shock proteins are RNA binding proteins involved in various cellular processes, including adaptation to low temperature, nutritional stress, cell growth and stationary phase. They may have an impact on gene expression by interfering with RNA stability and acting as transcription antiterminators. Caulobacter crescentus cspC is an essential gene encoding a stationary phase-induced protein of the Cold Shock Protein family and this work had as goal investigating the basis for the requirement of this gene for survival at this phase. In this work we investigate the role of CspC in C. crescentus stationary phase and discuss the molecular mechanisms that could be involved. Results: The expression of cspC increased significantly at stationary phase in complex media and in glucose depletion, indicating a putative role in responding to carbon starvation. Global transcriptional profiling experiments comparing cspC and the wild type strain both at exponential and stationary phases as well as comparing exponential and stationary phase in wild type strain were carried out by DNA microarray analysis. The results showed that the absence of cspC affected the transcription of 11 genes at exponential phase and 60 genes at stationary phase. Among the differentially expressed genes it is worth noting those encoding respiratory enzymes and genes for sulfur metabolism, which were upregulated, and those encoding enzymes of the glyoxylate cycle, which were severely downregulated in the mutant at stationary phase. mRNA decay experiments showed that the aceA mRNA, encoding isocitrate lyase, was less stable in the cspC mutant, indicating that this effect was at least partially due to posttranscriptional regulation. These observations were supported by the observed arrested growth phenotype of the cspC strain when grown in acetate as the sole carbon source, and by the upregulation of genes for assimilatory sulfate reduction and methionine biosynthesis. Conclusions: The stationary phase-induced RNA binding protein CspC has an important role in gene expression at this phase, and is necessary for maximal expression of the glyoxylate cycle genes. In the case of aceA, its downregulation may be attributed to the shorter half-life of the mRNA in the cspC mutant, indicating that one of the possible regulatory mechanisms is via altering RNA stabilization. (AU)

Processo FAPESP: 11/21883-2 - Caracterização dos mecanismos de ação da proteína cspC na manutenção da viabilidade e na resposta de Caulobacter crescentus a estresses
Beneficiário:Juliana Santos Nakayama
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 09/52883-8 - Caracterização de fatores sigma de função extracitoplasmática envolvidos na resposta a carência de ferro em Caulobacter crescentus
Beneficiário:Heloise Balhesteros
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/10563-0 - Sistemas regulatórios e fisiológicos da resposta bacteriana a estresses
Beneficiário:Marilis Do Valle Marques
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/04046-8 - Sistemas regulatórios da resposta bacteriana a estresses
Beneficiário:Marilis Do Valle Marques
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/17513-5 - Determinação dos genes regulados pelo fator de transcrição spdR e seu envolvimento na adaptação a fase estacionária em Caulobacter crescentus
Beneficiário:Carolina Antunes Do Prado Tavares da Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/50604-4 - Caracterização bioquímica e estrutural das interações da proteína Rrp43 com outras subunidades do exossomos de levedura
Beneficiário:Rogério Ferreira Lourenço
Linha de fomento: Bolsas no Brasil - Pós-Doutorado