Decay kinetics of HIV-1 specific T cell responses in vertically HIV-1 exposed seronegative infants

Autor(es)

Holditch, Sara J. ^[1] ; Eriksson, Emily M. ^[1] ; Tarosso, Leandro F. ^[2] ; Kuebler, Peter J. ^[1] ; Kallas, Esper G. ^[2] ; Nielsen, Erik K. ^[3] ; Wiznia, Andrew A. ^[3] ; Rosenberg, Michael G. ^[3] ; Nixon, Douglas F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calif San Francisco, San Francisco Gen Hosp, Dept Med, Div Expt Med, San Francisco, CA - USA

^[2] Univ Sao Paulo, Sch Med, Div Clin Immunol & Allergy, Sao Paulo - Brazil

^[3] Albert Einstein Coll Med, Jacobi Med Ctr, Bronx, NY 10467 - USA

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 2, 2012.
• Citações Web of Science: 4

Resumo

Objective: The majority of infants born, in developed countries, to HIV-1 positive women are exposed to the HIV-1 virus in utero or peri/post-partum, but are born uninfected.We, and others, have previously shown HIV-1 specific T cell responses in HIV-1 exposed seronegative (HESN) neonates/infants. Our objective in this study was to examine the rate of decay in their HIV-1 specific T cell response over time from birth. Design: Cross-sectional and longitudinal studies of HIV-1 specific T cell responses in HESN infants were performed. Methods: Peripheral blood mononuclear cells (PBMC) were isolated from 18 HIV-1 DNA PCR negative infants born to HIV-1 infected mothers receiving care at the Jacobi Medical Center, Bronx, NY, USA. PBMC were examined for T cell responses to HIV-1 antigens by interferon-gamma (IFN-gamma) ELISPOT. Results: PBMC from 15 HESN neonates/infants were analyzed. We observed a decay of HIV-1 specific T cell responses from birth at a rate of -0.599 spot forming unit/10(6) cells per day, with a median half-life decay rate of 21.38 weeks (13.39-115.8). Conclusion: Our results support the dynamic nature of T cell immunity in the context of a developing immune system. The disparate rate of decay with studies of adults placed on antiretroviral drugs suggests that antigen specific T cell responses are driven by the natural rate of decay of the T cell sub-populations themselves. (AU)

• Processo FAPESP: 10/05845-0 - Resposta imune celular em doenças infecciosas e imunodeficiências primárias
• Beneficiário: Esper Georges Kallás
• Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Linha de fomento: Auxílio à Pesquisa - Temático