Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix

Autor(es)

Souza, Talita F. B. ^[1] ; Sakamoto, Silmara S. ^[1] ; Ferreira, Gabriel T. N. M. ^[1] ; Gameiro, Roberto ^[2] ; Marinho, Marcia ^[2] ; de Andrade, Alexandre L. ^[1] ; Cardoso, Tereza C. ^[2]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Coll Vet Med, Small Anim Surg Ctr, BR-16050680 Aracatuba, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Coll Vet Med, Lab Anim Virol & Cell Culture, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Veterinary Science; v. 16, n. 3, p. 381-384, SEP 2015.
• Citações Web of Science: 3

Resumo

Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after-14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro. (AU)

• Processo FAPESP: 10/52465-9 - Isolamento e caracterização genética do reovirus de perus (TReoV) provenientes de quadros de enterite associados a PEMS (Poult Enteritis Mortality Syndrome) no Brasil
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50845-1 - Isolamento e caracterização genética do reovírus de Peru de amostras clínicas provenientes de quadro de entrerite associados a PEMS no Brasil
• Beneficiário: Ana Carolina Guedes Rosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado