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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix

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Author(s):
Souza, Talita F. B. [1] ; Sakamoto, Silmara S. [1] ; Ferreira, Gabriel T. N. M. [1] ; Gameiro, Roberto [2] ; Marinho, Marcia [2] ; de Andrade, Alexandre L. [1] ; Cardoso, Tereza C. [2]
Total Authors: 7
Affiliation:
[1] UNESP Univ Estadual Paulista, Coll Vet Med, Small Anim Surg Ctr, BR-16050680 Aracatuba, SP - Brazil
[2] UNESP Univ Estadual Paulista, Coll Vet Med, Lab Anim Virol & Cell Culture, BR-16050680 Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Veterinary Science; v. 16, n. 3, p. 381-384, SEP 2015.
Web of Science Citations: 3
Abstract

Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after-14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro. (AU)