Expansion of mesenchymal stromal cells derived from human umbilical cord matrix in bioreactors in serum/xeno-free conditions

• Grant number: 13/23599-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): March 01, 2014
• Effective date (End): October 31, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Kamilla Swiech Antonietto
• Grantee: Amanda Mizukami Martins
• Supervisor: Cláudia Lobato da Silva
• Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil
• Research place: Universidade de Lisboa, Portugal
• Associated to the scholarship: 12/23228-4 - In vitro expansion of mesenchymal stromal cells and secretome characterization: therapeutic and biotechnological applications, BP.DR

Abstract

The mesenchymal stromal cells ( MSCs ) have become of great interest for cell therapy because of their potential to differentiate and replenish specialized tissues . Can be isolated from diverse tissues, but the umbilical cord matrix (UCM) is a promising source of MSCs for clinical applications , because the cord is usually discarded and its collection is non- invasive . However, the number of MSCs isolated from a single cord blood unit is restricted and the need for expansion is essential to achieve cell numbers adequate for a therapeutic application. The cell expansion using bioreactor technology offers the right tools to develop and optimize a culture system at low cost , with monitoring and control of the cultivation parameters , for the expansion of human MSCs quickly , safely and effectively . Furthermore, the use of reagents with undefined compositions and components derived from animals such as fetal bovine serum ( FBS ) introducing into the culture medium high concentrations of proteins that may be allergenic / immunogenic and increase the risk of contamination affecting the use of MSCs for therapeutic purposes. Thus, although widely used to supplement the culture media, such components must be replaced with serum/xeno free media. In this context , the main objective is to establish an efficient bioprocess expansion of MSCs derived from umbilical cord in bioreactors under conditions of serum/xeno-free culture, both in terms of growth and for cell recovery. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIZUKAMI, AMANDA; PEREIRA CHILIMA, TANIA D.; ORELLANA, MARISTELA D.; ABREU NETO, MARIO; COVAS, DIMAS T.; FARID, SUZANNE S.; SWIECH, KAMILLA. Technologies for large-scale umbilical cord-derived MSC expansion: Experimental performance and cost of goods analysis. Biochemical Engineering Journal, v. 135, p. 36-48, JUL 15 2018. (13/23599-5, 13/08135-2, 12/23228-4)

MIZUKAMI, AMANDA; FERNANDES-PLATZGUMMER, ANA; CARMELO, JOANA G.; SWIECH, KAMILLA; COVAS, DIMAS T.; CABRAL, JOAQUIM M. S.; DA SILVA, CLAUDIA L.. Stirred tank bioreactor culture combined with serum-/xenogeneic-free culture medium enables an efficient expansion of umbilical cord-derived mesenchymal stem/stromal cells. Biotechnology Journal, v. 11, n. 8, p. 1048-1059, AUG 2016. (13/08135-2, 12/23228-4, 13/23599-5)