Low expression of Gria1 and Grin1 glutamate receptors in the nucleus accumbens of Spontaneously Hypertensive Rats (SHR)

Autor(es)

Diana, Mariana C. ^{[1, 2]} ; Santoro, Marcos L. ^{[3, 1]} ; Xavier, Gabriela ^[3] ; Santos, Camila Mauricio ^{[1, 2]} ; Spindola, Leticia N. ^{[3, 1]} ; Moretti, Patricia N. ^{[3, 1]} ; Ota, Vanessa K. ^{[3, 1]} ; Bressan, Rodrigo A. ^[1] ; Abilio, Vanessa C. ^{[1, 2]} ; Belangero, Sintia I. ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psychiat, LiNC Interdisciplinary Lab Clin Neurosci, BR-05039032 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Pharmacol, BR-04039032 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Morphol & Genet, Div Genet, BR-04023900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PSYCHIATRY RESEARCH; v. 229, n. 3, p. 690-694, OCT 30 2015.
• Citações Web of Science: 5

Resumo

The Spontaneously Hypertensive Rat (SHR) strain is a classical animal model for the study of essential hypertension. Recently, our group suggested that this strain could be a useful animal model for schizophrenia, which is a severe mental illness with involvement of glutamatergic system. The aim of this study is to investigate glutamatergic receptors (Gria1 and Grin1) and glycine transporter (Glyt1) gene expression in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) of SHR animals. The effects in gene expression of a chronic treatment with antipsychotic drugs (risperidone, haloperidol and clozapine) were also analyzed. Animals were treated daily for 30 days, and euthanized for brain tissue collection. The expression pattern was evaluated by Real Time Reverse-Transcriptase (RT) PCR technique. In comparison to control rats, SHR animals present a lower expression of both NMDA (Grin1) and AMPA (Gria1) gene receptors in the NAcc. Antipsychotic treatments were not able to change gene expressions in any of the regions evaluated. These findings provide evidence for the role of glutamatergic changes in schizophrenia-like phenotype of the SHR strain. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/08968-6 - Investigação de marcadores genéticos e epigenéticos: uma abordagem translacional para o tratamento de esquizofrenia
• Beneficiário: Síntia Iole Nogueira Belangero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/13859-1 - Análise da expressão de genes de receptores e reguladores de neurotransmissores em modelo animal para esquizofrenia
• Beneficiário: Marcos Leite Santoro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/50740-5 - Prevenção na esquizofrenia e no transtorno bipolar da neurociência à comunidade: uma plataforma multifásica, multimodal e translacional para investigação e intervenção
• Beneficiário: Rodrigo Affonseca Bressan
• Modalidade de apoio: Auxílio à Pesquisa - Temático