Low expression of Gria1 and Grin1 glutamate receptors in the nucleus accumbens of Spontaneously Hypertensive Rats (SHR)

Author(s)

Diana, Mariana C. ^{[1, 2]} ; Santoro, Marcos L. ^{[3, 1]} ; Xavier, Gabriela ^[3] ; Santos, Camila Mauricio ^{[1, 2]} ; Spindola, Leticia N. ^{[3, 1]} ; Moretti, Patricia N. ^{[3, 1]} ; Ota, Vanessa K. ^{[3, 1]} ; Bressan, Rodrigo A. ^[1] ; Abilio, Vanessa C. ^{[1, 2]} ; Belangero, Sintia I. ^{[3, 1]}

Affiliation

^[1] Univ Fed Sao Paulo, Dept Psychiat, LiNC Interdisciplinary Lab Clin Neurosci, BR-05039032 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Pharmacol, BR-04039032 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Morphol & Genet, Div Genet, BR-04023900 Sao Paulo - Brazil

• Document type: Journal article
• Source: PSYCHIATRY RESEARCH; v. 229, n. 3, p. 690-694, OCT 30 2015.
• Web of Science Citations: 5

Abstract

The Spontaneously Hypertensive Rat (SHR) strain is a classical animal model for the study of essential hypertension. Recently, our group suggested that this strain could be a useful animal model for schizophrenia, which is a severe mental illness with involvement of glutamatergic system. The aim of this study is to investigate glutamatergic receptors (Gria1 and Grin1) and glycine transporter (Glyt1) gene expression in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) of SHR animals. The effects in gene expression of a chronic treatment with antipsychotic drugs (risperidone, haloperidol and clozapine) were also analyzed. Animals were treated daily for 30 days, and euthanized for brain tissue collection. The expression pattern was evaluated by Real Time Reverse-Transcriptase (RT) PCR technique. In comparison to control rats, SHR animals present a lower expression of both NMDA (Grin1) and AMPA (Gria1) gene receptors in the NAcc. Antipsychotic treatments were not able to change gene expressions in any of the regions evaluated. These findings provide evidence for the role of glutamatergic changes in schizophrenia-like phenotype of the SHR strain. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 10/08968-6 - Investigation of genetic and epigenetic markers: a translational approach for schizophrenia treatment
• Grantee: Síntia Iole Nogueira Belangero
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/13859-1 - Analysis of gene expression of receptors and regulators of neurotransmitters in an animal model for schizophrenia
• Grantee: Marcos Leite Santoro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/50740-5 - Prevention in schizophrenia and bipolar disorder from neuroscience to the community: a multiphase, multimodal and translational platform for research and intervention
• Grantee: Rodrigo Affonseca Bressan
• Support Opportunities: Research Projects - Thematic Grants