Absorption of PCB126 by upper airways impairs G protein-coupled receptor-mediated immune response

Autor(es)

Shimada, Ana Lucia B. ^[1] ; Cruz, Wesley S. ^[1] ; Loiola, Rodrigo A. ^[1] ; Drewes, Carine C. ^[1] ; Doerr, Fabiane ^[1] ; Figueiredo, Natalia G. ^[1] ; Pinto, Ernani ^[1] ; Farsky, Sandra H. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Analyses, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 5, OCT 9 2015.
• Citações Web of Science: 4

Resumo

PCB126 is a dioxin-like polychlorinated biphenyl (PCB) environmental pollutant with a significant impact on human health, as it bioaccumulates and causes severe toxicity. PCB126-induced immune toxicity has been described, although the mechanisms have not been fully elucidated. In this study, an in vivo protocol of PCB126 intoxication into male Wistar rats by intranasal route was used, which has not yet been described. The intoxication was characterised by PCB126 accumulation in the lungs and liver, and enhanced aryl hydrocarbon receptor expression in the liver, lungs, kidneys, and adipose tissues. Moreover, an innate immune deficiency was characterised by impairment of adhesion receptors on blood leukocytes and by reduced blood neutrophil locomotion and oxidative burst activation elicited by ex vivo G protein-coupled receptor (GPCR) activation. Specificity of PCB126 actions on the GPCR pathway was shown by normal burst oxidative activation evoked by Toll-like receptor 4 and protein kinase C direct activation. Moreover, in vivo PCB180 intoxication did not alter adhesion receptors on blood leukocytes either blood neutrophil locomotion, and only partially reduced the GPCR-induced burst oxidative activation on neutrophils. Therefore, a novel mechanism of in vivo PCB126 toxicity is described which impairs a pivotal inflammatory pathway to the host defence against infections. (AU)

• Processo FAPESP: 12/02994-0 - Estudo experimental da exposição ao PCB126 sobre a indução de Diabetes mellitus tipo II
• Beneficiário: Sandra Helena Poliselli Farsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/09677-8 - Estudo experimental da exposição ao PCB126 sobre a indução de Diabetes Mellitus tipo II
• Beneficiário: Ana Lucia Borges Shimada
• Modalidade de apoio: Bolsas no Brasil - Doutorado