Gene expression analysis in blood of ultra-high risk subjects compared to first-episode of psychosis patients and controls

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Santoro, Marcos Leite ^{[1, 2]} ; Gadelha, Ary ^{[2, 3]} ; Ota, Vanessa K. ^{[1, 2]} ; Cunha, Graccielle R. ^[3] ; Asevedo, Elson ^[3] ; Noto, Cristiano S. ^{[4, 3]} ; Spindola, Leticia M. ^{[1, 2]} ; Pan, Pedro M. ^{[2, 3]} ; Talarico, Fernanda ^[1] ; Mansur, Rodrigo B. ^{[2, 3]} ; Silva, Patricia N. ^{[1, 2]} ; Brietzke, Elisa ^{[2, 3]} ; Cordeiro, Quirino ^[4] ; Bressan, Rodrigo A. ^{[2, 3]} ; Belangero, Sintia Iole ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Morphol & Genet, Div Genet, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, LiNC Interdisciplinary Lab Clin Neurosci, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, PRISMA Program Recognit & Intervent Subjects At R, Sao Paulo - Brazil

^[4] Irmandade Santa Casa Misericordia Sao Paulo, Dept Psychiat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF BIOLOGICAL PSYCHIATRY; v. 16, n. 6, p. 441-446, 2015.
• Citações Web of Science: 6

Resumo

Objectives. This study aimed to investigate peripheral blood gene expression in ultra-high-risk subjects (UHR) compared to first-episode psychosis individuals (FEP) and healthy controls (HC). Methods. We enrolled 22 UHR, 66 FEP and 67 HC and investigated the expression of 12 genes using Taqman assays. We used the Univariate General Linear Model, as well as Bonferroni correction for multiple comparisons. Results. We found that UFD1L (ubiquitin fusion degradation 1 like (yeast)) gene was upregulated in UHR group compared to HC and FEP (P = 3.44 x 10(-6); P = 9.41 x 10(-6)). MBP (myelin basic protein) was downregulated in UHR compared to FEP (P = 6.07 x 10(-6)). DISC1 (disrupted in schizophrenia 1) was also upregulated in UHR compared to FEP but lost statistical significance when corrected for age. Conclusions. These genes are directly related to neurodevelopmental processes and have been associated to schizophrenia. Recent findings described that DISC1 overexpression can disrupt MBP expression, thus, we think that these alterations in UHR individuals could be associated with a common process. UFD1L showed a different pattern of expression only for UHR group, suggesting that they can be under an acute endoplasmatic reticulum stress, demanding elevated levels of Ufd1. Further studies can improve knowledge on disease progression and putative targets to preventive strategies. (AU)

• Processo FAPESP: 10/08968-6 - Investigação de marcadores genéticos e epigenéticos: uma abordagem translacional para o tratamento de esquizofrenia
• Beneficiário: Síntia Iole Nogueira Belangero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/12686-1 - Busca por potenciais marcadores genéticos nas fases iniciais da esquizofrenia
• Beneficiário: Marcos Leite Santoro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50740-5 - Prevenção na esquizofrenia e no transtorno bipolar da neurociência à comunidade: uma plataforma multifásica, multimodal e translacional para investigação e intervenção
• Beneficiário: Rodrigo Affonseca Bressan
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/19176-3 - Análise de expressão gênica e metilação de DNA de pacientes em primeiro episódio psicótico virgens de tratamento
• Beneficiário: Vanessa Kiyomi Ota Kuniyoshi
• Modalidade de apoio: Bolsas no Brasil - Doutorado