Comparative testis morphology of Neotropical anurans

Autor(es)

Leite, Gabriela Baroni ^[1] ; Franco-Belussi, Lilian ^[2] ; Provete, Diogo B. ^[3] ; de Oliveira, Classius ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Biol, Grad Program Anim Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Fed Goias, Inst Biol Sci, Dept Ecol, Grad Program Ecol & Evolut, BR-74001970 Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZOOLOGISCHER ANZEIGER; v. 257, p. 29-38, 2015.
• Citações Web of Science: 2

Resumo

Anuran testes have generally similar germinative and somatic anatomical components, but their morphology varies among species groups. However, little is known about how environmental and evolutionary factors influence testis shape and sperm quantity. Here, we tested the hypothesis that the arrangement of both germinal compartment (seminiferous locules containing germ cells) and somatical compartment (interstitial fibers and somatic cells) differ between species with distinct reproductive modes and breeding activities. We compared the testes of eight species from two families of Neobatrachian anurans: Physalaemus centralis, Physalaemus cuvieri, Physalaemus marmoratus, Physalaemus olfersii, Pseudopaludicola sp. (aff. murundu) (Leptodactylidae: Leiuperinae), Crossodactylus caramaschii, Hylodes cardosoi, and Hylodes sazimai (Hylodidae). We built a 3D model of the testis to visualize the morphology and arrangement of seminiferous locules. We measured locular diameter and area, thickness of reticular fiber bundles, and area of both collagen fibers and sperm bundles. Hylodids had the highest values for most characters, except for sperm and reticular fiber bundles. For example, their locular diameter was 50%, and locular area 20% larger than leiuperines, which usually had thicker reticular fibers bundles. These fibers provide greater stability to the large quantity of small, spherical seminiferous locules found in leiuperines. Conversely, hylodids had larger area of collagen fibers, which provides greater flexibility and resistance to a few large and flattened seminiferous lad. Our results can shed light on how testicular morphology is influenced by evolutionary processes and ecological strategies, such as breeding seasonality and reproductive modes in anurans. (C) 2015 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 13/02067-5 - Efeitos tóxicos dos herbicidas Atrazina e Roundup em Eupemphix nattereri (Leiuperidae) e Rhinella schneideri (Bufonidae): alterações morfológicas sob diferentes concentrações e condições abióticas (pH, temperatura e fotoperíodo)
• Beneficiário: Classius de Oliveira
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/00946-4 - Pigmentação Visceral em Animais Ectotérmicos: Imunidade Inata e Defesa contra UV
• Beneficiário: Lilian Franco Belussi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/07284-4 - Morfologia testicular de anuros: análise comparativa em dez espécies do grupo Calamitophrynia
• Beneficiário: Gabriela Baroni Leite
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/16900-3 - Efeitos da alta temperatura na pigmentação visceral e no epitélio germinativo masculino em Eupemphix nattereri e Leptodactylus fuscus (Anura)
• Beneficiário: Gabriela Baroni Leite
• Linha de fomento: Bolsas no Brasil - Mestrado