Small Rho GTPases are important for acinus formation in a human salivary gland cell line

Autor(es)

Crema, Virginia O. ; Hamassaki, Dania E. ; Santos, Marinilce F.

• Tipo de documento: Artigo Científico
• Fonte: Cell and Tissue Research; v. 325, n. 3, p. 493-500, SEP 2006.
• Citações Web of Science: 11

Resumo

Rho GTPases participate in a wide variety of signal transduction pathways regulating the actin cytoskeleton, gene expression, cellular migration and proliferation. The aim of this study was to evaluate the role of Rho GTPases in signal transduction pathways during acinus formation in a human salivary gland (HSG) cell line initiated by extracellular matrix (ECM; Matrigel) alone or in combination with epidermal growth factor, basic fibroblast growth factor and lysophosphatidic acid (LPA). Immunohistochemical and Western blotting analyses showed that HSG cells contained RhoA, RhoB, Rac1 and Cdc42 proteins. All growth factors enhanced the effects of ECM on acinus formation, in a pathway dependent on PI3-kinase and Rho GTPases. The role of ROCK, a major RhoA effector, seemed limited to cortical actin polymerization. LPA stimulated cell migration and acinus formation in a PI3-kinase-independent pathway. The results suggest that Rho proteins are important for epithelial-mesenchymal interactions during salivary gland development. (AU)

• Processo FAPESP: 01/09047-2 - Desenvolvimento e degeneração da retina de vertebrados: aspectos celulares e moleculares
• Beneficiário: Dania Emi Hamassaki
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 97/09507-6 - Estudo da expressao de diferentes receptores para matriz extracelular durante a morfogenese e diferenciacao da glandula submandibular de ratos.
• Beneficiário: Marinilce Fagundes dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular