Mathematical modeling of enzyme production using Trichoderma harzianum P49P11 and sugarcane bagasse as carbon source

Autor(es)

Gelain, Lucas ^[1] ; da Cruz Pradella, Jose Geraldo ^[2] ; da Costa, Aline Carvalho ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, BR-13083852 Sao Paulo - Brazil

^[2] Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083852 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 198, p. 101-107, DEC 2015.
• Citações Web of Science: 2

Resumo

A mathematical model to describe the kinetics of enzyme production by the filamentous fungus Trichoderma harzianum P49P11 was developed using a low cost substrate as main carbon source (pre-treated sugarcane bagasse). The model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40 g L-1). The 10 g L-1 concentration was used to validate the model and the other to parameter estimation. The model for enzyme production has terms implicitly representing induction and repression. Substrate variation was represented by a simple degradation rate. The models seem to represent well the kinetics with a good fit for the majority of the assays. Validation results indicate that the models are adequate to represent the kinetics for a biotechnological process. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/19271-1 - Modelagem matemática da produção de enzimas celulolíticas por fungos filamentosos em fermentação submersa
• Beneficiário: Lucas Gelain
• Modalidade de apoio: Bolsas no Brasil - Mestrado