Mathematical modeling of enzyme production using Trichoderma harzianum P49P11 and sugarcane bagasse as carbon source

Author(s)

Gelain, Lucas ^[1] ; da Cruz Pradella, Jose Geraldo ^[2] ; da Costa, Aline Carvalho ^[1]

Affiliation

^[1] Univ Estadual Campinas, BR-13083852 Sao Paulo - Brazil

^[2] Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083852 Sao Paulo - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 198, p. 101-107, DEC 2015.
• Web of Science Citations: 2

Abstract

A mathematical model to describe the kinetics of enzyme production by the filamentous fungus Trichoderma harzianum P49P11 was developed using a low cost substrate as main carbon source (pre-treated sugarcane bagasse). The model describes the cell growth, variation of substrate concentration and production of three kinds of enzymes (cellulases, beta-glucosidase and xylanase) in different sugarcane bagasse concentrations (5; 10; 20; 30; 40 g L-1). The 10 g L-1 concentration was used to validate the model and the other to parameter estimation. The model for enzyme production has terms implicitly representing induction and repression. Substrate variation was represented by a simple degradation rate. The models seem to represent well the kinetics with a good fit for the majority of the assays. Validation results indicate that the models are adequate to represent the kinetics for a biotechnological process. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/19271-1 - Mathematical modelling of cellulolitic enzymes production by filamentous fungi in submerse fermentation
• Grantee: Lucas Gelain
• Support Opportunities: Scholarships in Brazil - Master