Production of cellulases and xylanases in different lignocellulosic substrates by axenic submerged cultivation of Trichoderma reesei QM9414 in mixed culture with Trichoderma harzianum FS09

• Grant number: 14/09457-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2014
• Effective date (End): August 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Pedro de Oliva Neto
• Grantee: Ludmila Melo Hergesel
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

Due to the wide availability of lignocellulosic wastes which are often discarded, and are increasingly being quoted as a big bet in use in biotechnological processes due to the low economic cost and reduced environmental impact. These wastes are being studied worldwide for the production of higher value-added compounds, such as enzymes, fermentable sugars, and bioenergy. The objective of this work is to develop a bioprocess using different types of waste which aim to produce cellulases and xylenes, aiming future applications in biotechnological processes. The process occurs through the submerged cultivation in a simple batch with Trichoderma reesei QM9414 and mixed culture with Trichoderma harzianum FS09. The methodology that is going to be employed is the use of various agro residues: bagasse from sugar cane, napier grass (Pennisetum purpureum), citrus pulp and cotton cake (pre-treatment or in natura), as a carbon source for the formulation of a culture medium that stimulates enzyme production.