Optimization of fibrolytic fungal enzymes production from agroindustrial residues and its application in ethanol industry

• Grant number: 17/03126-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2017
• End date: November 30, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Pedro de Oliva Neto
• Grantee: Natalie Stephanie Sawada Nunes
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

The residues generated from agricultural products are rich in cellulose, hemicellulose and lignin. These raw materials can serve as substrate to obtain fibrolytic enzymes, through bioprocesses performed by selected microorganisms. Some culture parameters as formulation of culture media, economical and efficient, from fibrolytic residues to optimize the enzyme production process are important for the value added to them. The enzymes are applied in the hydrolysis of the cell wall to the yeasts for deflocculation, without affecting the viability and decreasing its negative effect on fermentation. A statistical design of mixtures is used (60-100% mushroom production residue, 0-40% citrus pulp and 0-20% wheat bran), which has a greater influence on the production of xylanases and cellulases, mannanase, protease, from submerged fermentation of the residues with the fungus Trichoderma reesei QM9414. The production of fungal enzymes using residue from the mushroom production is not found in the literature, as well as its use in the hydrolysis of the cell wall of yeasts is little studied. (AU)