Effect of 4-(N,N-dimethylamino)phenethyl Alcohol on Degree of Conversion and Cytotoxicity of Photo-Polymerized CQ-Based Resin Composites

Autor(es)

Dayane Carvalho Ramos Salles de Oliveira ^[1] ; Camila Batista da Silva ^[2] ; Bruno Vilela Muniz ^[3] ; Maria C. Volpato ^[4] ; Ana Rosa Costa ^[5] ; Mario Alexandre Coelho Sinhoreti ^[6]

Afiliação do(s) autor(es)

^[1] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[2] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Physiological Sciences - Brasil

^[3] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Physiological Sciences - Brasil

^[4] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Physiological Sciences - Brasil

^[5] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[6] UNICAMP - University of Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 25, n. 6, p. 538-542, 2014-12-00.

Resumo

The aim of this study was to evaluate the degree of conversion (DC) and the cytotoxicity of photo-cured experimental resin composites containing 4-(N,N-dimethylamino)phenethyl alcohol (DMPOH) combined to the camphorquinone (CQ) compared with ethylamine benzoate (EDAB). The resin composites were mechanically blended using 35 wt% of an organic matrix and 65 wt% of filler loading. To this matrix was added 0.2 wt% of CQ and 0.2 wt% of one of the reducing agents tested. 5x1 mm samples (n=5) were previously submitted to DC measurement and then pre-immersed in complete culture medium without 10% (v/v) bovine serum for 1 h or 24 h at 37 °C in a humidifier incubator with 5% CO2 and 95% humidity to evaluate the cytotoxic effects of experimental resin composites using the MTT assay on immortalized human keratinocytes cells. As a result of absence of normal distribution, the statistical analysis was performed using the nonparametric Kruskal-Wallis to evaluate the cytotoxicity and one-way analysis of variance to evaluate the DC. For multiple comparisons, cytotoxicity statistical analyses were submitted to Student-Newman-Keuls and DC analysis to Tukey's HSD post-hoc test (=0.05). No significant differences were found between the DC of DMPOH (49.9%) and EDAB (50.7%). 1 h outcomes showed no significant difference of the cell viability between EDAB (99.26%), DMPOH (94.85%) and the control group (100%). After 24 h no significant difference were found between EDAB (48.44%) and DMPOH (38.06%), but significant difference was found compared with the control group (p>0.05). DMPOH presented similar DC and cytotoxicity compared with EDAB when associated with CQ. (AU)

• Processo FAPESP: 13/04241-2 - Influência do sistema fotoiniciador e agentes redutores nas propriedades físicas de resinas experimentais fotoativadas por LEDs
• Beneficiário: Dayane Carvalho Ramos Salles de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado