Chronic ethanol consumption induces erectile dysfunction: Role of oxidative stress

Autor(es)

Muniz, Jaqueline J. ^[1] ; Leite, Leticia N. ^{[1, 2]} ; De Martinis, Bruno S. ^[3] ; Carneiro, Fernando S. ^[2] ; Tirapelli, Carlos R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Enfermagem Ribeirao Preto, Lab Farmacol, BR-14040902 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Farmacol, BR-14040902 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciancias & Letras Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 141, p. 44-53, NOV 15 2015.
• Citações Web of Science: 11

Resumo

Aims: Investigate the effects of chronic ethanol consumption on erectile function and on the corpus cavernosum (CC) reactivity to endothelin-1 (ET-1). Main methods: Male Wistar rats were treated with ethanol (20% v/v) for six weeks. Key findings: Ethanol-treated rats showed impaired erectile function represented by decreased intracavernosal pressure/mean arterial pressure (ICP/MAP) responses. Ethanol consumption increased the contractile response induced by ET-1 in the isolated CC. Tiron increased ET-1-induced contraction in CC from control and ethanol-treated rats. No differences in the maximal contraction to ET-1 were observed after incubation of CC with PEG-catalase. SG560 and SC236 increased ET-1-induced contraction in CC from ethanol-treated rats. Y27632 reduced the contraction induced by ET-1 in CC from control and ethanol-treated rats. Ethanol increased plasma TBARS, superoxide anion (O-2(-)) levels and intracellular reactive oxygen species (ROS) generation in the rat CC. Reduced hydrogen peroxide (H2O2) levels in CC and increased catalase (CAT) activity in plasma and CC were detected after treatment with ethanol. Ethanol decreased superoxide dismutase (SOD) activity in the rat CC Increased expression of COX-1 was observed in CC from ethanol-treated rats. Treatment with ethanol decreased COX-2 expression but did not alter the expression of Nox1, RhoA and p-RhoA (ser(188)) in the rat CC. Significance: The major new findings of our study are that ethanol consumption induces erectile dysfunction (ED) and increases the contraction induced by ET-1 in the rat CC by a mechanism that involves decreased generation of H2O2 and vasodilator prostanoids as well as increased activation of the RhoA/Rho-kinase pathway. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/04144-4 - Consequências do consumo crônico de etanol sobre a via das metaloproteinases e MAPKs e sobre o remodelamento tecidual em corpo cavernoso de ratos.
• Beneficiário: Jaqueline Jóice Muniz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/12911-2 - Consequências do consumo crônico de etanol sobre a reatividade e expressão dos componentes do sistema endotelinérgico em corpo cavernoso de rato
• Beneficiário: Carlos Renato Tirapelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular