Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism

Autor(es):Mostrar menos -

de Oliveira, Amanda Priscila ^[1] ; Bernardo, Cassia Rubia ^[1] ; da Silveira Camargo, Ana Vitoria ^[1] ; Ronchi, Luiz Sergio ^[2] ; Borim, Aldenis Albaneze ^{[2, 3]} ; Brandao de Mattos, Cinara Cassia ^[1] ; de Campos Junior, Eumildo ^{[2, 3]} ; Castiglioni, Lilian ^[4] ; Netinho, Joao Gomes ^{[2, 3]} ; Cavasini, Carlos Eugonio ^[5] ; Bestetti, Reinaldo Bulgarelli ^[6] ; de Mattos, Luiz Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose do Rio Preto, Dept Biol Mol, Lab Imunogenet, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose do Rio Preto, Dept Cirugia, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Hosp Base, Fundacao Fac Reg Med, Dept Cirurgia Geral, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[4] Fac Med Sao Jose do Rio Preto, Dept Epidemiol & Saude Colet, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[5] Fac Med Sao Jose do Rio Preto, Ctr Invest Microrganismos, Dept Doencas Dermatol Infecciosas & Parasitarias, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[6] Fac Med Sao Jose do Rio Preto, Dept Cardiol & Cirurgia Cardiovasc, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 11 NOV 23 2015.
• Citações Web of Science: 12

Resumo

The clinical manifestations of chronic Chagas disease include the cardiac form of the disease and the digestive form. Not all the factors that act in the variable clinical course of this disease are known. This study investigated whether the CCR5 Delta 32 (rs333) and CCR5 59029 A/G (promoter region-rs1799987) polymorphisms of the CCR5 gene are associated with different clinical forms of chronic Chagas disease and with the severity of left ventricular systolic dysfunction in patients with chronic Chagas heart disease (CCHD). The antibodies anti-T. cruzi were identified by ELISA. PCR and PCR-RFLP were used to identify the CCR5.32 and CCR5 59029 A/G polymorphisms. The chi-square test was used to compare variables between groups. There was a higher frequency of the AA genotype in patients with CCHD compared with patients with the digestive form of the disease and the control group. The results also showed a high frequency of the AG genotype in patients with the digestive form of the disease compared to the other groups. The results of this study show that the CCR5.32 polymorphism does not seem to influence the different clinical manifestations of Chagas disease but there is involvement of the CCR5 59029 A/G polymorphism in susceptibility to the different forms of chronic Chagas disease. Besides, these polymorphisms do not influence left ventricular systolic dysfunction in patients with CCHD. (AU)

• Processo FAPESP: 11/19439-7 - Marcadores imunogenéticos relacionados à doença de Chagas
• Beneficiário: Ana Vitória da Silveira Camargo
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/20735-2 - Marcadores imunogenéticos relacionados à doença de Chagas
• Beneficiário: Ana Vitória da Silveira Camargo
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 11/08075-4 - Marcadores imunogenéticos relacionados à doença de Chagas
• Beneficiário: Luiz Carlos de Mattos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/05580-2 - Marcadores imunogenéticos relacionados à doença de Chagas
• Beneficiário: Cássia Rubia Bernardo
• Modalidade de apoio: Bolsas no Brasil - Mestrado