Osteogenic markers are reduced in bone-marrow mesenchymal cells and femoral bone of young spontaneously hypertensive rats

Autor(es)

de Barros, Thamine Landim ^{[1, 2]} ; Balera Brito, Victor Gustavo ^[1] ; Ferreira do Amaral, Caril Constante ^{[1, 2]} ; Chaves-Neto, Antonio Hernandes ^[1] ; Campanelli, Ana Paula ^[3] ; Penha Oliveira, Sandra Helena ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Sch Dent Aracatuba, Dept Basic Sci, Campus Aracatuba, Sao Paulo - Brazil

^[2] UNESP Univ Estadual Paulista, Sch Dent Aracatuba, Dept Basic Sci, Programa Posgrad Multictr Ciencias Fisiol SBFIS F, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, Campus Bauru, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 146, p. 174-183, FEB 1 2016.
• Citações Web of Science: 6

Resumo

Aims: Spontaneously hypertensive rats (SHR) and normotensive rats (W) has significant changes in bone metabolism. The purpose of this study was to investigate whether, the genetic predisposition, is sufficient to induce changes in the osteoblast differentiation and osteogenic markers in the BMSCs or in the femoral bone. For this we use young SHR rats without hypertension, but, with genetic predisposition in compared with young W. Main methods: BMSCs were cultured in a proliferation medium (MEM) or osteogenic medium. Osteogenic differentiation was analyzed by proliferation, total protein, alkaline phosphatase, mineralization, and the mRNA expression of RUNX-2, beta-cathenin, osterix, bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), bone sialoprotein (BSP), collagen type I (Col I), and osteopontin (OPN). Key findings: Osteoblast differentiation in SHR BMSCs (SHRC) had an increased proliferation compared with W BMSCs (WC). After osteogenic induction, there was greater reduction in proliferation in SHR (SHROM) than in W, in the same condition (WOM). On day 7, although no significant difference in the ALP activity was observed between SHROM and WOM, poor mineralization and osteoblast differentiation was noted in SHROM. The Osterix and beta-catenin are involved in the reduced osteoblast differentiation in SHROM. The decreased expression of osteoblast-associated proteins such as OCN, BSP, COL I and OPN revealed poor quality of extracellular matrix (ECM) in SHROM. In the femoral bone, the immunostaining of COL1, BALP, OPN and OCN in SHR was decreased compared with the W. TRAP-positive immunoreactions were observed in major extension in the SHR femur. Significance: This study is the first to compare osteoblast differentiation in vitro and femoral bone from SHR and W rats. Our results demonstrated that young SHR (4 weeks old), without hypertension, but with genetic predisposition, had alterations in osteoblast differentiation of BMSCs and in the femoral bone when compared with their progenitor strain, W. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/01924-9 - Estudo dos mecanismos envolvidos no processo de diferênciação em linhagem osteogênica de células-tronco mesenquimais da medula óssea de ratos Wistar e ratos espontaneamente hipertensos(SHR)
• Beneficiário: Thamine Landim de Barros
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/06070-5 - Estudo do fenótipo osteoblástico em células-tronco mesenquimais de ratos normotensos e espontaneamente hipertensos (SHR) na presença ou não de doença periodontal
• Beneficiário: Sandra Helena Penha de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/19458-1 - Avaliação dos efeitos da alta concentração de glicose e da participação da via insulínica sobre a diferenciação in vitro em linhagem osteogênica de células-tronco mesenquimais da medula óssea de ratos espontaneamente hipertensos
• Beneficiário: Sandra Helena Penha de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular