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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Osteogenic markers are reduced in bone-marrow mesenchymal cells and femoral bone of young spontaneously hypertensive rats

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Author(s):
de Barros, Thamine Landim [1, 2] ; Balera Brito, Victor Gustavo [1] ; Ferreira do Amaral, Caril Constante [1, 2] ; Chaves-Neto, Antonio Hernandes [1] ; Campanelli, Ana Paula [3] ; Penha Oliveira, Sandra Helena [1, 2]
Total Authors: 6
Affiliation:
[1] UNESP Univ Estadual Paulista, Sch Dent Aracatuba, Dept Basic Sci, Campus Aracatuba, Sao Paulo - Brazil
[2] UNESP Univ Estadual Paulista, Sch Dent Aracatuba, Dept Basic Sci, Programa Posgrad Multictr Ciencias Fisiol SBFIS F, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, Campus Bauru, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Life Sciences; v. 146, p. 174-183, FEB 1 2016.
Web of Science Citations: 6
Abstract

Aims: Spontaneously hypertensive rats (SHR) and normotensive rats (W) has significant changes in bone metabolism. The purpose of this study was to investigate whether, the genetic predisposition, is sufficient to induce changes in the osteoblast differentiation and osteogenic markers in the BMSCs or in the femoral bone. For this we use young SHR rats without hypertension, but, with genetic predisposition in compared with young W. Main methods: BMSCs were cultured in a proliferation medium (MEM) or osteogenic medium. Osteogenic differentiation was analyzed by proliferation, total protein, alkaline phosphatase, mineralization, and the mRNA expression of RUNX-2, beta-cathenin, osterix, bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), bone sialoprotein (BSP), collagen type I (Col I), and osteopontin (OPN). Key findings: Osteoblast differentiation in SHR BMSCs (SHRC) had an increased proliferation compared with W BMSCs (WC). After osteogenic induction, there was greater reduction in proliferation in SHR (SHROM) than in W, in the same condition (WOM). On day 7, although no significant difference in the ALP activity was observed between SHROM and WOM, poor mineralization and osteoblast differentiation was noted in SHROM. The Osterix and beta-catenin are involved in the reduced osteoblast differentiation in SHROM. The decreased expression of osteoblast-associated proteins such as OCN, BSP, COL I and OPN revealed poor quality of extracellular matrix (ECM) in SHROM. In the femoral bone, the immunostaining of COL1, BALP, OPN and OCN in SHR was decreased compared with the W. TRAP-positive immunoreactions were observed in major extension in the SHR femur. Significance: This study is the first to compare osteoblast differentiation in vitro and femoral bone from SHR and W rats. Our results demonstrated that young SHR (4 weeks old), without hypertension, but with genetic predisposition, had alterations in osteoblast differentiation of BMSCs and in the femoral bone when compared with their progenitor strain, W. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/19458-1 - Analysis of the effect of high glucose concentration and participation of insulin pathways on mesenchymal stem cells differentiation into osteoblastic lineages from bone marrow of spontaneously hypertensive rats
Grantee:Sandra Helena Penha de Oliveira
Support type: Regular Research Grants
FAPESP's process: 11/06070-5 - Study of osteoblatic phenotype from bone-marrow stem cells from normotensive and spontaneously hypertensive rats (SHR) in the presence or not of periodontal disease
Grantee:Sandra Helena Penha de Oliveira
Support type: Regular Research Grants
FAPESP's process: 12/01924-9 - Study of the mechanisms involved in the process of differentiation into osteogenic lineage of mesenchymal stem cells from bone marrow of Wistar rats and spontaneously hypertensive rats (SHR)
Grantee:Thamine Landim de Barros
Support type: Scholarships in Brazil - Master