|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||May 01, 2012|
|Effective date (End):||February 28, 2014|
|Field of knowledge:||Health Sciences - Dentistry|
|Principal researcher:||Sandra Helena Penha de Oliveira|
|Grantee:||Thamine Landim de Barros|
|Home Institution:||Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil|
Osteoporosis is a bone disorder that leads to reduction in bone mass and increased risk of fractures. Hypertension is a multifactorial disease, determined by genetic and environmental factors, considered a risk factor for osteoporosis. The pharmacological therapies available for the management of osteoporosis have no effect on hypertension related to osteoporosis. SHR (spontaneously hypertensive rats) are a genetic model of hypertension that showed damage bone architecture. Hyperproliferative activity and changes in insulin signaling pathway in smooth muscle cells from rat aorta SHR of 4 weeks of age has seen the influence of hypertensive genotype in cell biology of these animals, however it is unknown whether this condition can affect bone metabolism. Therefore, this study will evaluate whether the hypertensive genotype may influence the differentiation into osteogenic lineage of mesenchymal stem cells of young rats SHR (4 weeks) have not yet developed hypertension, in order to show only the influence of genotype hypertensive. To answer this question will be assessed the following parameters: gene expression of osteogenic transcription factors (Runx2 and ²-catenin) and bone matrix proteins collagen (type I collagen) and non-collagen (bone sialoprotein, osteopontin, osteocalcin); cell proliferation, total protein, alkaline phosphatase activity, formation of mineralized matrix, and modulating the activity of the signaling pathway of protein kinases activated by mitogenic agents (MAPKs - ERK 1/2, p38 and SAPK / JNK).