Study of osteoblatic phenotype from bone-marrow stem cells from normotensive and spontaneously hypertensive rats (SHR) in the presence or not of periodontal disease

• Grant number: 11/06070-5
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2011
• End date: May 31, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Sandra Helena Penha de Oliveira
• Grantee: Sandra Helena Penha de Oliveira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Antonio Hernandes Chaves Neto

Abstract

Systemic diseases have been associated with episodes of hypertension, such as diabetes, hyperthyroidism, dyslipidemia and osteoporosis. The purpose of this study is to evaluate the development potential of the osteoblastic phenotype of mesenchymal stem cells derived from bone marrow of normotensive and hypertensive rats in the presence or absence of an inflammatory process. For this we use the colorimetric MTT assay (3 - [4,5-dimethylthiazol-2-yl] -2.5 - diphenyl tetrazolium bromide), measurement of total protein, measurement of alkaline phosphatase, detection and quantification of extracellular matrix, elisaimunoensaio, and Real-Time PCR (Polymerase Chain Reactions), and immunohistochemical analysis. Will be used 20 rats divided into 2 groups: normotensive and spontaneously hypertensive (SHR), and these will be subdivided into two other subgroups: presence or absence of periodontal disease. The animals will be euthanized 15 days after the ligation, and this time will be made to collect the bone marrow of the femurs of the animals. Stem cells are deposited in the appropriate culture medium until reaching confluence. The regions undergoing periodontal bandage will be removed for laboratory processing and paraffin embedding. Some series of sections will be stained with hematoxylin and eosin to perform histomorphometric analysis. The analysis will be performed through optical microscopy bright field, and the data will be analyzed statistically. Photographs of the slides are captured with the aid of a camera attached to a stereomicroscope. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BARROS, THAMINE LANDIM; BALERA BRITO, VICTOR GUSTAVO; FERREIRA DO AMARAL, CARIL CONSTANTE; CHAVES-NETO, ANTONIO HERNANDES; CAMPANELLI, ANA PAULA; PENHA OLIVEIRA, SANDRA HELENA. Osteogenic markers are reduced in bone-marrow mesenchymal cells and femoral bone of young spontaneously hypertensive rats. Life Sciences, v. 146, p. 174-183, FEB 1 2016. (12/01924-9, 11/06070-5, 11/19458-1)

CHAVES NETO, ANTONIO H.; BRITO, VICTOR G. B.; LANDIM DE BARROS, THAMINE; DO AMARAL, CARIL C. F.; SUMIDA, DORIS H.; OLIVEIRA, SANDRA H. P.. Chronic high glucose and insulin stimulate bone-marrow stromal cells adipogenic differentiation in young spontaneously hypertensive rats. Journal of Cellular Physiology, v. 233, n. 9, p. 6853-6865, SEP 2018. (11/06070-5, 11/19458-1, 11/02558-3)

BALERA BRITO, VICTOR GUSTAVO; CHAVES-NETO, ANTONIO HERNANDES; DE BARROSA, THAMINE LANDIM; PENHA OLIVEIRA, SANDRA HELENA. Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells. Journal of Ethnopharmacology, v. 244, NOV 15 2019. (11/06070-5, 11/19458-1, 12/20547-1)