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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dietary leucine supplementation minimises tumour-induced damage in placental tissues of pregnant, tumour-bearing rats

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Autor(es):
Gomes Cruz, Bread Leandro [1] ; da Silva, Priscila Cristina [1] ; Tomasin, Rebeka [1] ; Oliveira, Andre Gustavo [1] ; Viana, Lais Rosa [1] ; Salomao, Emilianne Miguel [1] ; Cintra Gomes-Marcondes, Maria Cristina [1, 2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, State Univ Campinas, Inst Biol, Dept Struct & Funct Biol, CP 6109, BR-13083862 Campinas, SP - Brazil
[2] Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BMC CANCER; v. 16, FEB 4 2016.
Citações Web of Science: 4
Resumo

Background: The occurrence of cancer during pregnancy merges two complex, poorly understood metabolic and hormonal conditions. This association can exacerbate the conditions of both the mother and the foetus. The branched-chain amino acid leucine enhances cellular activity, particularly by increasing protein synthesis. This study aimed to analyse the modulatory effect of a leucine-rich diet on direct and indirect tumour-induced placental damage. This was accomplished by evaluating the expression of genes involved in protein synthesis and degradation and assessing anti-oxidant enzyme activity in placental tissues collected from pregnant, tumour-bearing rats. Results: Pregnant rats were either implanted with Walker 256 tumour cells or injected with ascitic fluid (to study the indirect effects of tumour growth) and then fed a leucine-rich diet. Animals in a control group underwent the same procedures but were fed a normal diet. On the 20th day of pregnancy, tumour growth was observed. Dams fed a normoprotein diet showed the greatest tumour growth. Injection with ascitic fluid mimicked the effects of tumour growth. Decreased placental protein synthesis and increased protein degradation were observed in both the tumour-bearing and the ascitic fluid-injected groups that were fed a normoprotein diet. These effects resulted in low placental DNA and protein content and high lipid peroxidation (measured by malondialdehyde content). Decreased placental protein synthesis-related gene expression was observed in the tumour group concomitant with increased expression of genes encoding protein degradation-associated proteins and proteolytic subunits. Conclusions: Consumption of a leucine-rich diet counteracted the effects produced by tumour growth and injection with ascitic fluid. The diet enhanced cell signalling, ameliorated deficiencies in DNA and protein content, and balanced protein synthesis and degradation processes in the placenta. The improvements in cell signalling included changes in the mTOR/eIF pathway. In conclusion, consumption of a leucine-rich diet improved placental metabolism and cell signalling in tumour-bearing rats, and these changes reduced the deleterious effects caused by tumour growth. (AU)

Processo FAPESP: 13/16115-1 - Estudo dos mecanismos bioquímicos e moleculares da caquexia: avaliação dos efeitos do fator Walker (FW) e da leucina em cultura celular de miotúbulos (células C2C12)
Beneficiário:Maria Cristina Cintra Gomes Marcondes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/08276-0 - Ação in vivo de L-leucina sobre sinalização celular e vias metabólicas durante o metabolismo protéico muscular em ratos portadores de tumor Walker 256
Beneficiário:Bread Leandro Gomes da Cruz
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/00209-9 - Nutrição e câncer estudo das alterações metabólicas e moleculares do metabolismo protéico no câncer-caquexia, moduladas pela suplementação nutricional com leucina, no crescimento, desenvolvimento e gravidez
Beneficiário:Maria Cristina Cintra Gomes Marcondes
Linha de fomento: Auxílio à Pesquisa - Regular