Free-Ranging Frigates (Fregata magnificens) of the Southeast Coast of Brazil Harbor Extraintestinal Pathogenic Escherichia coli Resistant to Antimicrobials

Autor(es)

Saviolli, Juliana Yuri ^{[1, 2]} ; Vieira Cunha, Marcos Paulo ^[1] ; Lopes Guerra, Maria Flavia ^[1] ; Irino, Kinue ^[3] ; Catao-Dias, Jose Luiz ^[1] ; de Carvalho, Vania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet, Dept Pathol, Sao Paulo, SP - Brazil

^[2] Projeto Albatroz, Santos, SP - Brazil

^[3] Adolfo Lutz Inst, Enterobacteriacea Lab, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 2 FEB 4 2016.
• Citações Web of Science: 2

Resumo

Seabirds may be responsible for the spread of pathogenic/resistant organisms over great distances, playing a relevant role within the context of the One World, One Health concept. Diarrheagenic E. coli strains, known as STEC (shiga toxin-producing E. coli), and the extra-intestinal pathogenic E. coli (ExPEC and the subpathotype APEC), are among the E. coli pathotypes with zoonotic potential associated with the birds. In order to identify health threats carried by frigates and to evaluate the anthropic influence on the southern coast of Brazil, the aim of this work was to characterize E. coli isolated from free-ranging frigates in relation to virulence genotypes, serotypes, phylogenetic groups and antimicrobial resistance. Cloacal and choanal swabs were sampled from 38 Fregata magnificens from two oceanic islands and one rescue center. Forty-three E. coli strains were recovered from 33 out of the 38 birds (86.8%); 88.4% of strains showed some of the virulence genes (VGs) searched, 48.8% contained three or more VGs. None of the strains presented VGs related to EPEC/STEC. Some of the isolates showed virulence genotypes, phylogenetic groups and serotypes of classical human ExPEC or APEC (O2:H7, O1:H6, ONT:H7, O25:H4). Regarding antimicrobial susceptibility, 62.8% showed resistance, and 11.6% (5/43) were multidrug-resistant. The E. coli present in the intestines of the frigates may reflect the environmental human impact on southeast coast of Brazil; they may also represent an unexplored threat for seabird species, especially considering the overlap of pathogenic potential and antimicrobial resistance present in these strains. (AU)

• Processo FAPESP: 10/20706-7 - Pesquisa de fatores preditores de virulência para aves em cepas de ExPEC isoladas de aves de vida livre.
• Beneficiário: Marcos Paulo Vieira Cunha
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/51943-4 - Pesquisa de sensibilidade aos antimicrobianos em cepas de E. coli isoladas de aves de vida livre capturadas na costa Sudeste brasileira, com especial interesse na pesquisa de genes de resistência à tetraciclina
• Beneficiário: Vania Maria de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular