| Texto completo | |
| Autor(es): |
Lima, Cilene Reboucas
[1]
;
Geraldo, Murilo Vieira
[1]
;
Fuziwara, Cesar Seigi
[1]
;
Kimura, Edna Teruko
[1]
;
Santos, Marinilce Fagundes
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, Ave Prof Lineu Prestes 1524, Predio 1, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC CANCER; v. 16, FEB 16 2016. |
| Citações Web of Science: | 32 |
| Resumo | |
Background: Tumor invasiveness is directly related to the ability of tumor cells to migrate and invade surrounding tissues, usually degrading extracellular matrix. Despite significant progress in the knowledge about migration and invasion, there is much more to elucidate about their regulatory mechanisms, especially in cancer cells. MicroRNAs (miRs) were recently described as important regulators of migration. Differential expression of miRs in cancer is frequently associated with progression, invasion and metastasis. In papillary thyroid carcinoma (PTC), miR-146b-5p is highly expressed and positively correlated to the degree of malignancy. Methods: This study aimed to investigate the role of miR-146b-5p on the migratory and invasive behaviors of thyroid cells, using a non tumor rat thyroid follicular cell line (PCCl3) transfected with the miR-146b-5p genomic region, and two PTC cell lines (TPC-1 and BCPAP, bearing distinct oncogenic backgrounds), which express high levels of miR-146b-5p, after miR-146b inhibition by antagomiR and miR-146b overexpression by mimics-miR. Migration and invasion were studied by time-lapse and transwell assays (with and without Matrigel (R)). Gelatin degradation assays were also employed, as well as F-actin staining. Results: Migration and invasion of PCCl3 were increased 2-3x after miR-146b-5p overexpression (10X) and large lamellipodia were evident in those cells. After miR-146b-5p inhibition, TPC-1 and BCPAP migration and invasion were significantly reduced, with cells showing several simultaneous processes and low polarity. Gelatin degradation was inhibited in TPC-1 cells after inhibition of miR-146b-5p, but was unaffected in BCPAP cells, which did not degrade gelatin. The inhibition of miR-146b-5p in PCCl3 also inhibited migration and invasion, and additional (exogenous) overexpression of this miR in TPC-1 and BCPAP cells increased migration and invasion, without effects on cell morphology or gelatin degradation. The overexpression of SMAD4 in BCPAP cells, a validated target of miR-146b-5p and key protein in the TGF-beta signaling pathway, inhibited migration similarly to the effects observed with the antagomiR 146b-5p. Conclusions: miR-146b-5p positively regulates migration and invasion of thyroid normal and tumor follicular cells (independently from their original mutation, either BRAF or RET/PTC), through a mechanism that involves the actin cytoskeleton but not an increased capacity of matrix degradation. (AU) | |
| Processo FAPESP: | 11/18936-7 - MicroRNAs e invasividade tumoral: envolvimento de GTPases Rho |
| Beneficiário: | Cilene Rebouças de Lima |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/03990-9 - Regulação da migração celular por microRNAs: implicações no Diabetes mellitus, regeneração tecidual e câncer |
| Beneficiário: | Marinilce Fagundes dos Santos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |