Ovarian superstimulation using FSH combined with equine chorionic gonadotropin (eCG) upregulates mRNA-encoding proteins involved with LH receptor intracellular signaling in granulosa cells from Nelore cows

Autor(es)

Castilho, A. C. S. ^[1] ; Nogueira, M. F. G. ^[2] ; Fontes, P. K. ^[1] ; Machado, M. F. ^[1] ; Satrapa, R. A. ^[1] ; Razza, E. M. ^[1] ; Barros, C. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil

^[2] Univ Sao Paulo State, Dept Biol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 82, n. 9, p. 1199-1205, DEC 2014.
• Citações Web of Science: 3

Resumo

The LH plays a key role in controlling physiological processes in the ovary acting via LH receptor (LHR). In general, the effects of LHR on the regulation of granulosa cell differentiation are mediated mainly via the Gs-protein/adenylyl cyclase/cAMP system; however, the LHR activation could also induce phospholipase C (PLC)/inositol trisphosphate (IP3) via Gq/11 system. Additionally, the expression of G-proteins (GNAS, GNAQ and GNA11) and PLC beta has been showed in bovine antral follicle, concomitant with an increase in LHR expression. To gain insight into the effects of superstimulation with FSH (P-36 protocol) or FSH combined with equine chorionic gonadotropin (eCG; P-36/eCG protocol) on the mRNA expression of proteins involved in LHR signaling in bovine granulosa cells, Nelore cows (Bos indicus) were treated with two superstimulatory protocols: P-36 protocol or P-36/eCG protocol (replacement of the FSH by eCG administration on the last day of treatment). Nonsuperstimulated cows were only submitted to estrous synchronization without ovarian superstimulation. The granulosa cells were harvested from follicles and mRNA abundance of GNAS, GNAQ, GNA11, PLCB1, PLCB, PLCB4, and adenylyl cyclase isoforms (ADCY3, ADCY4, ADCY6, ADCY8, and ADGY9) was measured by real-time reserve transcription followed by polymerase chain reaction. No differences on mRNA abundance of target genes were observed in granulosa cells of cows submitted to P-36 protocol compared with control group. However, the cows submitted to P-36/eCG protocol showed upregulation on the mRNA abundance of target genes (except ADCY8) in granulosa cells. Although the P-36 protocol did not regulate mRNA expression of the proteins involved in the signaling mechanisms of the cAMP and IP3 systems, the constant presence of GNAS, GNAQ GNA11, PLCB1, PLCB3, PLCB4, and adenylyl cyclase isoforms (ADCY3, ADCY4, ADCY6, and ADCY9) mRNA and the upregulation of these genes in granulosa cells from cows submitted to P-36/eCG protocol reinforce the participation of G(q/11)/PLC/IP3 signaling as well as Gs-protein/adenylyl cyclase/cAMP system on LHR pathways during bovine granulosa cell differentiation submitted to superstimulatory treatments. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/23409-9 - Expressão gênica diferencial, análise ultraestrutural e avaliação do perfil lipídico de blastocistos bovinos produzidos in vitro a partir de oócitos maturados convencionalmente ou pelo sistema SPOM
• Beneficiário: Eduardo Montanari Razza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/09498-9 - Influência da raça (Nelore e Angus) e da população folicular sobre o perfil de genes diferencialmente expressos em ovidutos bovinos
• Beneficiário: Patricia Kubo Fontes
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/51789-5 - Expressao de genes associados a qualidade, desenvolvimento e competencia oocitaria e embrionaria inicial de femeas nelores submetidas ou nao a super estimulacao ovariana
• Beneficiário: Rafael Augusto Satrapa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/10737-8 - Comparação entre a maturação in vitro convencional e um sistema de simulação da maturação oocitária fisiológica (spom) em complexos cumulus-oócito bovinos
• Beneficiário: Mariana Fernandes Machado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/52250-2 - Identificacao, caracterizacao e estudos in vitro dos mecanismos de sinalizacao celular dos transcritos alternativos do receptor de lh (lhr) na especie bovina.
• Beneficiário: Anthony César de Souza Castilho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/50593-2 - Estudos de genes associados à qualidade, competência oocitária e embrionária inicial de fêmeas Nelores e Angus submetidas ou não à superestimulação ovariana e dos mecanismos envolvidos na sinalização intracelular das diferentes isoformas de LHR na espécie bovina
• Beneficiário: Ciro Moraes Barros
• Modalidade de apoio: Auxílio à Pesquisa - Regular