Effects of Sleep Deprivation on Mice Bone Marrow and Spleen B Lymphopoiesis

Autor(es)

Lungato, Lisandro ^[1] ; Nogueira-Pedro, Amanda ^[2] ; Dias, Carolina Carvalho ^[3] ; Paredes-Gamero, Edgar Julian ^{[3, 4]} ; Tufik, Sergio ^[1] ; D'Almeida, Vania ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psychobiol, Rua Napoleao Barros 925, 3rd Floor, BR-04024002 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Biophys, Rua Napoleao Barros 925, 3rd Floor, BR-04024002 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Biochem, Rua Napoleao Barros 925, 3rd Floor, BR-04024002 Sao Paulo, SP - Brazil

^[4] Univ Mogi das Cruzes, Ctr Interdisciplinar Invest Bioqum, Av Dr Candido Xavier de Almeida Souza, Mogi Das Cruzes, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 231, n. 6, p. 1313-1320, JUN 2016.
• Citações Web of Science: 3

Resumo

B lymphocytes are immune cells crucial for the maintenance and viability of the humoral response. Sleep is an essential event for the maintenance and integrity of all systems, including the immune system (IS). Thus, sleep deprivation (SD) causes problems in metabolism and homeostasis in many cell systems, including the IS. In this study, our goal was to determine changes in B lymphocytes from the bone marrow (BM) and spleen after SD. Three-month-old male Swiss mice were used. These mice were sleep deprived through the modified multiple platform method for different periods (24, 48, and 72h), whereas another group was allowed to sleep for 24h after 72h of SD (rebound group) and a third group was allowed to sleep normally during the entire experiment. After this, the spleen and BM were collected, and cell analyses were performed. The numbers of B lymphocytes in the BM and spleen were reduced by SD. Additionally, reductions in the percentage of lymphocyte progenitors and their ability to form colonies were observed. Moreover, an increase in the death of B lymphocytes from the BM and spleen was associated with an increase in oxidative stress indicators, such as DCFH-DA, CAT, and mitochondrial SOD. Rebound was not able to reverse most of the alterations elicited by SD. The reduction in B lymphocytes and their progenitors by cell death, with a concomitant increase in oxidative stress, showed that SD promoted a failure in B lymphopoiesis. J. Cell. Physiol. (c) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs