Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane

Autor(es)

Melloni, Maria Natalia G. ^[1] ; Melloni, Maria Leticia G. ^[1] ; Neuber, Ana Caroline ^[2] ; Perecin, Dilermando ^[1] ; Landell, Marcos G. de Andrade ^[2] ; Pinto, Luciana R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Campus Jaboticabal, Jaboticabal, SP - Brazil

^[2] Ctr Cana Inst Agron Campinas, Rodovia Antonio Duarte Nogueira, Km 321, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SUGAR TECH; v. 18, n. 2, p. 222-228, APR 2016.
• Citações Web of Science: 1

Resumo

Commercial sugarcane cultivars and wild species have a high ploidy level allied to a complex genome and usual chromosomal instability. There are few studies related to the standardization of cytogenetic techniques in sugarcane. This work tested the efficiency of three antimitotics (colchicine, 8-hydroxyquinoline, and trifuralin) at different concentrations on the quality of metaphases spreads from a commercial sugarcane cultivar (IAC911099-Saccharum spp.) and a wild sugarcane accession (Krakatau-S. spontaneum). Although all three antimitotics were efficient for obtaining metaphases, 8-hydroxyquinoline was the most efficient for both IAC911099 and Krakatau. The chromosome number of these two genotypes was inferred. The variation in chromosome number was 2n = 90-112 for IAC911099, with a statistical modal of 2n = 112 chromosomes while the variation in chromosome number was 2n = 90-129 chromosome with a statistical modal of 2n = 128 chromosomes for Krakatau (S. spontaneum). (AU)

• Processo FAPESP: 12/15281-2 - "Caracterização molecular, citogenética e fenotípica de acessos do "Complexo Saccharum" para fins de introgressão genética"
• Beneficiário: Maria Natália Guindalini Melloni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático