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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane

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Author(s):
Melloni, Maria Natalia G. [1] ; Melloni, Maria Leticia G. [1] ; Neuber, Ana Caroline [2] ; Perecin, Dilermando [1] ; Landell, Marcos G. de Andrade [2] ; Pinto, Luciana R. [2]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Campus Jaboticabal, Jaboticabal, SP - Brazil
[2] Ctr Cana Inst Agron Campinas, Rodovia Antonio Duarte Nogueira, Km 321, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SUGAR TECH; v. 18, n. 2, p. 222-228, APR 2016.
Web of Science Citations: 1
Abstract

Commercial sugarcane cultivars and wild species have a high ploidy level allied to a complex genome and usual chromosomal instability. There are few studies related to the standardization of cytogenetic techniques in sugarcane. This work tested the efficiency of three antimitotics (colchicine, 8-hydroxyquinoline, and trifuralin) at different concentrations on the quality of metaphases spreads from a commercial sugarcane cultivar (IAC911099-Saccharum spp.) and a wild sugarcane accession (Krakatau-S. spontaneum). Although all three antimitotics were efficient for obtaining metaphases, 8-hydroxyquinoline was the most efficient for both IAC911099 and Krakatau. The chromosome number of these two genotypes was inferred. The variation in chromosome number was 2n = 90-112 for IAC911099, with a statistical modal of 2n = 112 chromosomes while the variation in chromosome number was 2n = 90-129 chromosome with a statistical modal of 2n = 128 chromosomes for Krakatau (S. spontaneum). (AU)

FAPESP's process: 12/15281-2 - Molecular, cytogenetic and phenotypic characterization of accessions from the Saccharum Complex for genetic introgression
Grantee:Maria Natália Guindalini Melloni
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 08/57908-6 - National Institute of Science and Technology of Bioethanol
Grantee:Marcos Silveira Buckeridge
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants