Radiation-synthesized protein-based drug carriers: Size-controlled BSA nanoparticles

Autor(es)

Queiroz, R. G. ^[1] ; Varca, G. H. C. ^[1] ; Kadlubowski, S. ^[2] ; Ulanski, P. ^[2] ; Lugao, A. B. ^[1]

Afiliação do(s) autor(es)

^[1] CNEN SP, IPEN, Ave Prof Lineu Prestes, 2242, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[2] Lodz Univ Technol, Inst Appl Radiat Chem, Wroblewskiego 15, PL-93590 Lodz - Poland

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 85, p. 82-91, APR 2016.
• Citações Web of Science: 7

Resumo

Nanotechnology has broadened the options for the delivery of agents of biotechnological and clinical relevance. Currently, attention has been driven towards the development of protein-based nanocarriers due to high the biocompatibility and site-specific delivery. In this work we report radiation-synthesized bovine serum albumin nanoparticles as an attempt to overcome limitations of available albumin particles, as a novel route for the development of crosslinked protein nanocarriers for the administration of chemotherapic agents or radiopharmaceuticals. Albumin containing phosphate buffer solutions were irradiated using gamma-irradiation at distinct cosolvent concentrations-ethanol or methanol. Nanoparticle size was followed by DLS and bityrosine crosslinking formation using fluorescence measurements and SDS-PAGE. In addition, computational experiments were performed to elucidate the mechanism and pathways for the nanoparticle formation. The synthesis of BSA nanoparticles using gamma-irradiation in the presence of a cosolvent allowed the formation of the nanoparticles from 7 to 70 nm without the use of any chemical crosslinker as confirmed by SDS-PAGE and DLS analysis. The combination of cosolvent and gamma-irradiation allowed a fine tuning with regard to protein size. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/13979-0 - Sistemas nanoestruturados à base de proteínas induzidos por radiação como carreadores de quimioterápicos
• Beneficiário: Gustavo Henrique Costa Varca
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado