Proteomics of Secretory-Stage and Maturation-Stage Enamel of Genetically Distinct Mice

Autor(es):Mostrar menos -

Charone, Senda ^[1] ; Leite, Aline De Lima ^[1] ; Peres-Buzalaf, Camila ^[1] ; Fernandes, Mileni Silva ^[1] ; de Almeida, Lucas Ferreira ^[1] ; Zardin Graeff, Marcia Sirlene ^[1] ; de Oliveira, Rodrigo Cardoso ^[1] ; Campanelli, Ana Paula ^[1] ; Groisman, Sonia ^[2] ; Whitford, Gary Milton ^[3] ; Everett, Eric T. ^[4] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Sch Dent, Community Dent, Rio De Janeiro - Brazil

^[3] Georgia Regents Univ, Dept Oral Biol, Coll Dent Med, Augusta, GA - USA

^[4] Univ N Carolina, Sch Dent, Dept Pediat Dent, Carolina Ctr Genome Sci, Chapel Hill, NC - USA

• Tipo de documento: Artigo Científico
• Fonte: Caries Research; v. 50, n. 1, p. 24-31, 2016.
• Citações Web of Science: 8

Resumo

The mechanisms by which excessive ingestion of fluoride (F) during amelogenesis leads to dental fluorosis (DF) are still not precisely known. Inbred strains of mice vary in their susceptibility to develop DF, and therefore permit the investigation of underlying molecular events influencing DF severity. We employed a proteomic approach to characterize and evaluate changes in protein expression from secretory-stage and maturation-stage enamel in 2 strains of mice with different susceptibilities to DF (A/J, i.e. `susceptible' and 129P3/J, i.e. `resistant'). Weanling male and female susceptible and resistant mice fed a low-F diet were divided into 2 F-water treatment groups. They received water containing 0 (control) or 50 mg F/l for 6 weeks. Plasma and incisor enamel was analyzed for F content. For proteomic analysis, the enamel proteins extracted for each group were separated by 2-dimensional electrophoresis and subsequently characterized by liquid-chromatography electrospray-ionization quadrupole time-of-flight mass spectrometry. F data were analyzed by 2-way ANOVA and Bonferroni's test (p < 0.05). Resistant mice had significantly higher plasma and enamel F concentrations when compared with susceptible mice in the F-treated groups. The proteomic results for mice treated with 0 mg F/l revealed that during the secretory stage, resistant mice had a higher abundance of proteins than their susceptible counterparts, but this was reversed during the maturation stage. Treatment with F greatly increased the number of protein spots detected in both stages. Many proteins not previously described in enamel (e.g. type 1 collagen) as well as some uncharacterized proteins were identified. Our findings reveal new insights regarding amelogenesis and how genetic background and F affect this process. (C) 2016 S. Karger AG, Basel (AU)

• Processo FAPESP: 08/03489-2 - Análise proteômica da matriz do esmalte de camundongos susceptíveis ou resistentes à fluorose dentária, expostos cronicamente a diferentes concentrações de F na água de beber
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/10589-6 - Análise proteômica da matriz do esmalte de camundongos susceptíveis ou resistentes à fluorose dentária, expostos cronicamente ou não ao flúor na água de beber
• Beneficiário: Senda Charone
• Modalidade de apoio: Bolsas no Brasil - Doutorado