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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response

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Autor(es):
Moreli, Jusciele B. [1] ; Santos, Janine H. [2] ; Lorenzon-Ojea, Aline Rodrigues [3] ; Correa-Silva, Simone [1, 3] ; Fortunato, Rodrigo S. [4] ; Rocha, Clarissa Ribeiro [5] ; Rudge, Marilza V. [1] ; Damasceno, Debora C. [1] ; Bevilacqua, Estela [3] ; Calderon, Iracema M. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Grad Program Gynecol Obstet & Mastol, Dist Rubiao Jr S-N, BR-18618000 Botucatu, SP - Brazil
[2] NIEHS, Mol Carcinogenesis Lab, Res Triangle Pk, NC - USA
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-09500900 Sao Paulo - Brazil
[4] Univ Fed Rio de Janeiro, Carlos Chagas Filho Biophys Inst, Lab Mol Radiobiol, Rio De Janeiro - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, DNA Repair Lab, BR-09500900 Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Sciences; v. 12, n. 4, p. 466-477, 2016.
Citações Web of Science: 5
Resumo

Objective: Investigate the DNA damage and its cellular response in blood samples from both mother and the umbilical cord of pregnancies complicated by hyperglycemia. Methods: A total of 144 subjects were divided into 4 groups: normoglycemia (ND; 46 cases), mild gestational hyperglycemia (MGH; 30 cases), gestational diabetes mellitus (GDM; 45 cases) and type-2 diabetes mellitus (DM2; 23 cases). Peripheral blood mononuclear cell (PBMC) isolation and/or leukocytes from whole maternal and umbilical cord blood were obtained from all groups at delivery. Nuclear and mitochondrial DNA damage were measured by gene-specific quantitative PCR, and the expression of mRNA and proteins involved in the base excision repair (BER) pathway were assessed by real-time qPCR and Western blot, respectively. Apoptosis was measured in vitro experiments by caspase 3/7 activity and ATP levels. Results: GDM and DM2 groups were characterized by an increase in oxidative stress biomarkers, an increase in nuclear and mitochondrial DNA damage, and decreased expression of mRNA (APE1, POLa and FEN1) and proteins (hOGG1, APE1) involved in BER. The levels of hyperglycemia were associated with the in vitro apoptosis pathway. Blood levels of DNA damage in umbilical cord were similar among the groups. Newborns of diabetic mothers had increased expression of BER mRNA (APE1, POLa and FEN1) and proteins (hOGG1, APE1, POL beta and FEN1). A diabetes-like environment was unable to induce apoptosis in the umbilical cord blood cells. Conclusions: Our data show relevant asymmetry between maternal and fetal blood cell susceptibility to DNA damage and apoptosis induction. Maternal cells seem to be more predisposed to changes in an adverse glucose environment. This may be due to differential ability in upregulating multiple genes involved in the activation of DNA repair response, especially the BER mechanism. However if this study shows a more effective adaptive response by the fetal organism, it also calls for further studies to determine the limit of this response that definitely changes the fate of a fetus under these conditions of cellular stress. (AU)

Processo FAPESP: 11/13562-1 - Lesões no DNA e capacidade de resposta celular de gestantes e recém-nascidos em regime de hiperglicemia de intensidade variada
Beneficiário:Jusciele Brogin Moreli
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/23296-0 - Danos no DNA nuclear e mitocondrial em gestantes e recém-nascidos com hiperglicemia de intensidade variada
Beneficiário:Jusciele Brogin Moreli
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 11/18240-2 - Associação entre estresse oxidativo, lesões no DNA e capacidade de resposta celular em gestantes e recém-nascidos sob regime de hiperglicemia de intensidade variada
Beneficiário:Iracema de Mattos Paranhos Calderon
Linha de fomento: Auxílio à Pesquisa - Regular