Response of Cytokines and Hydrogen Peroxide to Sporothrix schenckii Exoantigen in Systemic Experimental Infection

Autor(es)

Geraldo Maia, Danielle Cardoso ^[1] ; Goncalves, Amanda Costa ^[1] ; Ferreira, Lucas Souza ^[1] ; Manente, Francine Alessandra ^[1] ; Portuondo, Deivys Leandro ^[1] ; Rebuglio Vellosa, Jose Carlos ^{[1, 2]} ; Polesi, Marisa Campos ^[1] ; Batista-Duharte, Alexander ^{[1, 3]} ; Carlos, Iracilda Zeppone ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Anal Clin, Rua Expedicionarios Brasil 1621, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Estadual Ponta Grossa, Setor Ciencias Biol & Saude, Dept Anal Clin & Toxicol, Ponta Grossa, PR - Brazil

^[3] Med Sci Univ, Toxicol & Biomed Ctr TOXIMED, Immunotoxicol Lab, Autopista Nacl Km 1 1-2, Santiago De Cuba 90400 - Cuba

• Tipo de documento: Artigo Científico
• Fonte: Mycopathologia; v. 181, n. 3-4, p. 207-215, APR 2016.
• Citações Web of Science: 11

Resumo

The response of hydrogen peroxide (H2O2) and cytokines during an experimental sporotrichosis in male Swiss mice was assessed over a period of 10 weeks by monitoring macrophage activation challenged with exoantigen (ExoAg) from the fungus Sporothrix schenckii. The studied endpoints were: H2O2 production, fungal burden at spleen, apoptosis in peritoneal macrophages, and IL-1 beta, IL-6, IL-2, IL-10 production. During the two first weeks of infection was observed low burden of yeast in spleen and high response of H2O2, IL-2, and IL-1 beta. The weeks of highest fungal burden (fourth-sixth) coincided with major apoptosis in peritoneal macrophages, normal production of IL-6 and lower production of H2O2, IL-2, and IL-1 beta, suggesting a role for these three last in the early control of infection. On the other hand, IL-1 beta (but not IL-6) was recovered since the sixth week, suggesting a possible role in the late phase of infection, contributing to the fungal clearance in conjunction with the specific mechanisms. The IL-10 was elevated until the sixth, principally in the second week. These results evidences that ExoAg is involved in the host immune modulation, influencing the S. Schenckii virulence, and its role is related with the time of the infection in the model used. (AU)

• Processo FAPESP: 09/11442-9 - Polarização de macrófagos durante a fase inicial e tardia da infecção por Sporothrix schenckii em modelo murino
• Beneficiário: Iracilda Zeppone Carlos
• Modalidade de apoio: Auxílio à Pesquisa - Regular