Cervicovaginal bacterial count and failure of metronidazole therapy for bacterial vaginosis

Autor(es)

Luchiari, Heloise R. ^[1] ; Ferreira, Carolina S. T. ^[1] ; Golim, Marjorie A. ^[2] ; Silva, Marcia G. ^[1] ; Marconi, Camila ^[3]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Botucatu Med Sch, Ctr Blood, Botucatu, SP - Brazil

^[3] UFPR Univ Fed Parana, Setor Ciencias Biol, Dept Basic Pathol, BR-81531980 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Gynecology & Obstetrics; v. 132, n. 3, p. 297-301, MAR 2016.
• Citações Web of Science: 1

Resumo

Objective: To evaluate whether total bacterial count in cervicovaginal fluid is associated with failure of metronidazole therapy for bacterial vaginosis. Methods: In a cross-sectional study, women attending a primary health center in Botucatu, Sao Paulo, Brazil, for routine cervical screening between September 2012 and October 2013 were enrolled. Women who tested positive for bacterial vaginosis (Nugent classification) were offered oral metronidazole. Women who completed metronidazole treatment and an equal number of control women with normal vaginal flora at initial screening were included in analyses of total bacterial count, assessed by flow cytometry of cervicovaginal fluid samples. Results: Of 287 women who enrolled, 49 were excluded because they tested positive for trichomoniasis, chlamydial endocervicitis, gonorrhea, or candidiasis. Among the remaining 238, 85 (35.7%) had bacterial vaginosis. Among 36 women evaluated at follow-up, 23 (63.9%) had successfully restored lactobacilli-dominant flora, 12 (33.3%) had persistent bacterial vaginosis, and 1 (2.8%) had vaginal candidiasis (excluded from flow cytometry). Total bacterial count did not differ between 35 women with bacterial vaginosis and 35 with normal vaginal flora (P = 0.62). Total bacterial count did not differ at enrollment between women who went onto have persistent bacterial vaginosis and those who had successful treatment (P = 0.78). Conclusion: Failure of oral metronidazole therapy for bacterial vaginosis was not associated with total bacterial count in cervicovaginal fluid. (C) 2015 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/16800-3 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/10403-2 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Camila Marconi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/01750-3 - Avaliação da relação entre a resposta ao tratamento da vaginose bacteriana e a contagem bacteriana total por citometria de fluxo
• Beneficiário: Heloise Ranucci Luchiari
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica