In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil

Autor(es)

Nogueira Brilhante, Raimunda Samia ^[1] ; Rodrigues, Anderson Messias ^[2] ; Costa Sidrim, Jose Julio ^[1] ; Gadelha Rocha, Marcos Fabio ^[1] ; Pereira, Sandro Antonio ^[3] ; Ferreira Gremiao, Isabella Dib ^[3] ; Pacheco Schubach, Tania Maria ^[3] ; de Camargo, Zoilo Pires ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Ceara, Specialized Med Mycol Ctr, Postgrad Program Med Microbiol, Fortaleza, Ceara - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Cellular Biol Div, Sao Paulo - Brazil

^[3] Fundacao Oswaldo Cruz, Evandro Chagas Natl Inst Infect Dis, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 54, n. 3, p. 275-279, MAR 2016.
• Citações Web of Science: 6

Resumo

Sporotrichosis is an important subcutaneous mycosis of humans and animals. Classically, the disease is acquired upon traumatic inoculation of Sporothrix propagules from contaminated soil and plant debris. In addition, the direct horizontal transmission of Sporothrix among animals and the resulting zoonotic infection in humans highlight an alternative and efficient rout of transmission through biting and scratching. Sporothrix brasiliensis is themost virulent species of the Sporothrix schenckii complex and is responsible for the long-lasting outbreak of feline sporotrichosis in Brazil. However, antifungal susceptibility data of animal-borne isolates is scarce. Therefore, this study evaluated the in vitro activity of amphotericin B, caspofungin, itraconazole, voriconazole, fluconazole, and ketoconazole against animal-borne isolates of S. brasiliensis. The susceptibility tests were performed through broth microdilution (M38-A2). The results show the relevant activity of itraconazole, amphotericin B, and ketoconazole against S. brasiliensis, with the following MIC ranges: 0.125-2, 0.125-4 and 0.0312-2 mu g/ml, respectively. Caspofungin was moderately effective, displaying higher variation in MIC values (0.25-64 mu g/ml). Voriconazole (2-64 mu g/ml) and fluconazole (62.5-500 mu g/ml) showed low activity against S. brasiliensis strains. This study contributed to the characterization of the in vitro antifungal susceptibility of strains of S. brasiliensis recovered from cats with sporotrichosis, which have recently been considered the main source of human infections. (AU)

• Processo FAPESP: 09/54024-2 - Biologia molecular e proteômica de fungos de interesse médico: Paracoccidioides brasiliensis e Sporothrix schenckii
• Beneficiário: Zoilo Pires de Camargo
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/07350-1 - Biologia do complexo Sporothrix schenckii: Análise de marcadores moleculares e proteômicos e o papel de moléculas antigênicas e antifúngicas na esporotricose
• Beneficiário: Anderson Messias Rodrigues
• Linha de fomento: Bolsas no Brasil - Doutorado