Diarrheagenic Escherichia coli pathotypes investigation revealed atypical enteropathogenic E-coli as putative emerging diarrheal agents in children living in Botucatu, SAo Paulo State, Brazil

Autor(es)

Dias, Regiane C. B. ^[1] ; dos Santos, Bruna C. ^[1] ; dos Santos, Luis F. ^[2] ; Vieira, Melissa A. ^[1] ; Yamatogi, Ricardo S. ^[1] ; Mondelli, Alessandro L. ^[3] ; Sadatsune, Terue ^[1] ; Sforcin, Jose M. ^[1] ; Gomes, Tania A. T. ^[4] ; Hernandes, Rodrigo T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Microbiol & Imunol, BR-18618970 Botucatu, SP - Brazil

^[2] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo - Brazil

^[3] Univ Estadual Paulista Julio Mesquita Filho UNESP, Fac Med, Dept Patol Clin, Botucatu, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APMIS; v. 124, n. 4, p. 299-308, APR 2016.
• Citações Web of Science: 15

Resumo

The aim of the present study was to investigate the prevalence of Diarrheagenic Escherichia coli (DEC) pathotypes, a leading cause of diarrhea worldwide, among diarrheal and healthy children, up to 5 years of age, living in the city of Botucatu, SAo Paulo, Brazil. DEC, investigated by PCR detection of virulence factor-encoding genes associated with the distinct pathotypes, was isolated from 18.0% of the patients, and 19.0% of the controls, with enteroaggregative E. coli (EAEC), the most frequent pathotype, being detected in equal proportion between patients and controls (10.0%). Among the enteropathogenic E. coli (EPEC) isolates, only one isolate was able to produce the localized adherence pattern to HeLa cells, being thus the only typical EPEC identified. All the remaining EPEC were classified as atypical (aEPEC), and detected in 8.0% and 8.5% of the patients and controls, respectively. Regarding the serotypes, 26.5% of the analyzed EPEC isolates belonged to classical EPEC-serogroups, and the only two STEC found were serotyped as O26:H11 (patient) and O119:H7 (control). Antimicrobial susceptibility tests revealed that 43.6%, 29.5% and 2.6% of the DEC isolates were resistant to ampicillin, cotrimoxazole and gentamicin, respectively. Our data indicate that EAEC remains prevalent among children living in Botucatu, and revealed atypical EPEC as emerging putative diarrheal agents in this geographical region. (AU)

• Processo FAPESP: 12/19077-0 - Prevalência e caracterização fenotípica e molecular de amostras de Escherichia coli diarreiogênica isoladas de crianças com e sem diarréia na cidade Botucatu, São Paulo
• Beneficiário: Rodrigo Tavanelli Hernandes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/05170-1 - Prevalência e caracterização de amostras de Escherichia coli diarreiogênica isoladas de crianças na cidade de Botucatu, São Paulo
• Beneficiário: Regiane Chrysostomo Bitencort Dias
• Modalidade de apoio: Bolsas no Brasil - Mestrado