Vestitol Isolated from Brazilian Red Propolis Inhibits Neutrophils Migration in the Inflammatory Process: Elucidation of the Mechanism of Action

Autor(es)

Franchin, Marcelo ^[1] ; Colon, David F. ^[2] ; Castanheira, Fernanda V. S. ^[2] ; da Cunha, Marcos G. ^[1] ; Bueno-Silva, Bruno ^[1] ; Alencar, Severino M. ^[3] ; Cunha, Thiago M. ^[2] ; Rosalen, Pedro L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Luiz de Queiroz Coll Agr, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 79, n. 4, p. 954-960, APR 2016.
• Citações Web of Science: 14

Resumo

Vestitol is an isoflavonoid isolated from Brazilian red propolis with potential anti-inflammatory activity. This study investigated the mechanism of action of vestitol on the modulation of neutrophil migration in the inflammatory process. Pre-treatment with vestitol at 1, 3, or 10 mg/kg reduced LPS- or mBSA-induced neutrophil migration and the release of CXCL1/KC and CXCL2/MIP-2 in vivo. Likewise, pretreatment with vestitol at 1, 3, or 10 mu M reduced the levels of CXCL1/KC and CXCL2/MIP-2 in macrophage supernatants in vitro. Moreover, the administration of vestitol (10 mg/kg) reduced leukocyte rolling and adherence in the mesenteric microcirculation of mice. The pre-treatment with vestitol (10 mg/kg) in iNOS(-/-) mice did not block its activity concerning neutrophil migration. With regard to the activity of vestitol on neutrophils isolated from the bone marrow of mice, there was a reduction on the chemotaxis of CXCL2/MIP-2 or LTB4-induced neutrophils and on calcium influx after pre-treatment with the compound at 3 or 10 mu M. There was no change in CXCR2 expression by neutrophils treated with vestitol at 10 mu M. These findings demonstrate that vestitol is a promising novel anti-inflammatory agent. (AU)

• Processo FAPESP: 12/22378-2 - Estudo in vivo e in vitro da atividade de compostos isolados de própolis brasileiras sobre a modulação da migração de neutrófilos no processo inflamatório
• Beneficiário: Pedro Luiz Rosalen
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/01365-0 - Estudo in vivo e in vitro da atividade de compostos isolados de própolis brasileiras sobre a modulação da migração de neutrófilos no processo inflamatório
• Beneficiário: Marcelo Franchin
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs