Beneficial effects of vitamin C treatment on pregnant rats exposed to formaldehyde: Reversal of immunosuppression in the offspring

Autor(es)

Ibrahim, Beatriz Silva ^[1] ; Barioni, Eric Diego ^[1] ; Heluany, Cintia ^[1] ; Braga, Tarcio Teodoro ^[2] ; Drewes, Carine Cristiane ^[1] ; Costa, Silvia Goes ^[3] ; Saraiva Camara, Niels Olsen ^[2] ; Poliselli Farsky, Sandra Helena ^[1] ; Lino-dos-Santos-Franco, Adriana ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil

^[3] Univ Nove Julho UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, Rua Vergueiro 239-245, BR-01504000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicology and Applied Pharmacology; v. 300, p. 77-81, JUN 1 2016.
• Citações Web of Science: 5

Resumo

Inhalation of formaldehyde (FA) during the pregnancy induces oxidative stress in the uterus, and here we hypothesized that this mechanism may be responsible for the impaired immune response detected in the offspring. In order to investigate the protective effects of Vitamin Con the oxidative stress induced by FA in the uterine microenvironment, pregnant Wistar rats were treated with vitamin C (150 mg/kg, gavage) or vehicle (distilled water, gavage) I h before FA exposure (0.92 mg/m(3), 1 h/day, 5 days/week), for 21 days, and the 30 days old offspring were submitted to LPS injection (Salmonella abortus equi, 5 mg/kg, i.p.). The enhanced gene expression of iNOS, COX-1 and COX-2 and decreased gene expression of SOD-2 in the uterus of FA exposed mothers was rescued by Vit C treatment. Moreover, vitamin C rescued the impaired immune response elicited by LPS in the offspring from FA exposed mothers, by increasing the number of blood and bone marrow leukocytes, and augmenting gene expression of IL-6 and reducing mRNA levels of IL-10 and IFN in the lungs. Vitamin C treatment did not rescue the impaired TLR4-NF-kB pathway in the lung of the offspring, suggesting that FA-induced uterine oxidative stress affects other inflammatory pathways activated by LPS in the offspring. Together, data obtained here confirm our hypothesis that FA-induced oxidative stress in the uterine microenvironment modifies the programming mechanisms of the immune defenses of offspring, leading to an impaired host defense. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/19708-3 - Avaliação da repercussão da exposição intrauterina ao formaldeído no desenvolvimento de infecção pulmonar na prole
• Beneficiário: Beatriz Silva Ibrahim
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/51711-9 - Exposição in utero à poluição ambiental e ocupacional e sua repercussão para o desencadeamento de inflamação alérgica pulmonar na prole: correlação com mecanismos epigenéticos
• Beneficiário: Adriana Lino dos Santos Franco
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores