Zika virus impairs growth in human neurospheres and brain organoids

Autor(es)

Garcez, Patricia P. ^{[1, 2]} ; Loiola, Erick Correia ^[2] ; da Costa, Rodrigo Madeiro ^[2] ; Higa, Luiza M. ^[3] ; Trindade, Pablo ^[2] ; Delvecchio, Rodrigo ^[3] ; Nascimento, Juliana Minardi ^{[4, 2]} ; Brindeiro, Rodrigo ^[3] ; Tanuri, Amilcar ^[3] ; Rehen, Stevens K. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio de Janeiro, Inst Biomed Sci, Rio De Janeiro - Brazil

^[2] DOr Inst Res & Educ IDOR, Rio De Janeiro - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Biol, Rio De Janeiro - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Science; v. 352, n. 6287, p. 816-818, MAY 13 2016.
• Citações Web of Science: 388

Resumo

Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased considerably in Brazil; however, causality between the viral epidemic and malformations in fetal brains needs further confirmation. We examined the effects of ZIKV infection in human neural stem cells growing as neurospheres and brain organoids. Using immunocytochemistry and electron microscopy, we showed that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and brain organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development. (AU)

• Processo FAPESP: 14/21035-0 - Proteômica quantitativa de linhagens neurais e organóides cerebrais derivados de células tronco de pluripotência induzida de pacientes com esquizofrenia
• Beneficiário: Juliana Minardi Nascimento
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado