A simple and high-resolution HPLC-PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies

Autor(es)

Couto, Rene O. ^[1] ; Cubayachi, Camila ^[1] ; Lopez, Renata F. V. ^[1] ; de Gaitani, Cristiane M. ^[1] ; Pedrazzi, Vinicius ^[2] ; de Freitas, Osvaldo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Odontol Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL CHROMATOGRAPHY; v. 30, n. 6, p. 857-866, JUN 2016.
• Citações Web of Science: 4

Resumo

A simple, isocratic, high-resolution and prompt HPLC-PDA method was developed and validated for the simultaneous quantification of prilocaine (PCL) and lidocaine (LCL) hydrochlorides in in vitro buccal iontophoresis-driven permeation studies. A reversed-phase C-18 column (250 mm x 4.6 mm, 3 mu m, 110 angstrom) was used for the chromatographic separation. The mobile phase contained acetonitrile: 0.1M sodium phosphate buffer, pH 7.0 (1:1, v/v), plus 0.05% (v/v) diethylamine. The isocratic flow rate was set at 1 mL/min and the detection wavelength was 203 nm. PCL and LCL eluted in 8.9 min and 13 min, respectively, and the system suitability parameters varied within an acceptable range. The method was selective, sensitive, precise, accurate and robust, producing a linear plot at the concentration range of 0.25 to 10 mu g/mL. The application of this method was demonstrated by a significant enhancement of the permeation of PCL and LCL with the application of iontophoresis (1 mA/cm(2) per 1 h) through isolated porcine esophageal epithelium. The amount of the drug retained in the epithelium also increased with the application of an electrical current. Copyright (C) 2015 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 12/07251-6 - Desenvolvimento de plataforma com sistemas mucoadesivos para liberação de fármacos na cavidade bucal
• Beneficiário: Osvaldo de Freitas
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/05644-0 - Desenvolvimento de sistemas semi-sólidos mucoadesivos para liberação de anestésico e aplicação de iontoforese na cavidade bucal
• Beneficiário: Camila Cubayachi
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/06167-1 - Desenvolvimento de filmes mucoadesivos para liberação de fármacos anestésicos na cavidade bucal
• Beneficiário: Renê Oliveira Do Couto
• Linha de fomento: Bolsas no Brasil - Doutorado