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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A simple and high-resolution HPLC-PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies

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Couto, Rene O. [1] ; Cubayachi, Camila [1] ; Lopez, Renata F. V. [1] ; de Gaitani, Cristiane M. [1] ; Pedrazzi, Vinicius [2] ; de Freitas, Osvaldo [1]
Total Authors: 6
[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Odontol Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOMEDICAL CHROMATOGRAPHY; v. 30, n. 6, p. 857-866, JUN 2016.
Web of Science Citations: 4

A simple, isocratic, high-resolution and prompt HPLC-PDA method was developed and validated for the simultaneous quantification of prilocaine (PCL) and lidocaine (LCL) hydrochlorides in in vitro buccal iontophoresis-driven permeation studies. A reversed-phase C-18 column (250 mm x 4.6 mm, 3 mu m, 110 angstrom) was used for the chromatographic separation. The mobile phase contained acetonitrile: 0.1M sodium phosphate buffer, pH 7.0 (1:1, v/v), plus 0.05% (v/v) diethylamine. The isocratic flow rate was set at 1 mL/min and the detection wavelength was 203 nm. PCL and LCL eluted in 8.9 min and 13 min, respectively, and the system suitability parameters varied within an acceptable range. The method was selective, sensitive, precise, accurate and robust, producing a linear plot at the concentration range of 0.25 to 10 mu g/mL. The application of this method was demonstrated by a significant enhancement of the permeation of PCL and LCL with the application of iontophoresis (1 mA/cm(2) per 1 h) through isolated porcine esophageal epithelium. The amount of the drug retained in the epithelium also increased with the application of an electrical current. Copyright (C) 2015 John Wiley \& Sons, Ltd. (AU)

FAPESP's process: 12/07251-6 - Development of platform with mucoadhesive systems for drug release on the buccal cavity
Grantee:Osvaldo de Freitas
Support type: Regular Research Grants
FAPESP's process: 12/05644-0 - Development of mucoadhesive semisolid systems for anesthetic release and iontophoresis application on the buccal cavity
Grantee:Camila Cubayachi
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/06167-1 - Development of mucoadhesive films for anesthetic release on the buccal cavity
Grantee:Renê Oliveira Do Couto
Support type: Scholarships in Brazil - Doctorate