Dexamethasone Regulates Macrophage and Cd4+Cd25+Cell Numbers in the Chicken Spleen

Autor(es)

Calefi, A. S. ^[1] ; Quinteiro-Filho, W. M. ^[1] ; Fukushima, A. R. ^[2] ; da Cruz, D. S. G. ^[1] ; de Siqueira, A. ^[2] ; Salvagni, F. A. ^[2] ; Namazu, L. B. ^[1] ; Gomes, C. O. M. S. ^[1] ; Ferreira, A. J. P. ^[1] ; Palermo Neto, J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Neuroimmunomodulat Res Grp, Dept Pathol, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Pathol, Sch Vet Med & Anim Sci, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Poultry Science; v. 18, n. 1, p. 93-99, JAN-MAR 2016.
• Citações Web of Science: 4

Resumo

Dexamethasone (DEX) is a corticoid hormone that is experimentally used to mimic the effects of increased levels of endogenous corticosterone observed during the stress response. Currently, stress is considered one of the major predisposing factors for diseases in the poultry industry. The aim of this study was to analyze the effects of DEX and/or of a 20-fold coccidial vaccine dose on leukocyte phenotypes in the spleen and cecal tonsils of chickens. Twenty specific-pathogen-free (SPF) Leghorn chickens were divided into four groups: a non-treated group (NT), a DEX-treated group (Dex), a vaccinated group (V) and a DEX-treated+vaccinated group (Dex+V). On experimental day (ED) 42, each bird in the vaccinated groups received a anti-coccidial vaccine. DEX was injected in the birds of the Dex and Dex+V groups (0.9 mg/kg) onED42 and ED45. The immunophenotyping was performed by flow cytometry analysis of splenocytes and cecal tonsils cells onED48. DEX treatment per se was unable to change CD4(+)CD8(+), CD4(+)CD8(+) and CD4(-)CD8(+) populations with TCR gamma delta or CD28 in the spleen, or macrophages and T lymphocytes in the cecal tonsils. V group birds presented higher numbers of splenic macrophages compared with those measured in the Dex+V group. The number of CD4(+)CD25(+) cells in the spleen of birds of the V group was higher than those measured in the other experimental groups. Our data suggest that CD4(+)CD25(+) cells and macrophages might be influenced by DEX treatment in spleen, but not in the cecal tonsils of chickens inoculated with Eimeria. (AU)

• Processo FAPESP: 13/17408-2 - Avaliação dos efeitos do estresse por calor sobre a imunidade de frangos de corte em um modelo experimental de enterite necrótica aviária
• Beneficiário: Atilio Sersun Calefi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/03103-2 - Avaliação do estresse por calor sobre o desenvolvimento da enterite necrótica por Clostridium perfringens em frangos de corte
• Beneficiário: Atilio Sersun Calefi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/51886-3 - Neuroimunomodulação: fármacos, estresse e citocinas nas relações entre os sistemas nervoso, endócrino e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático