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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dexamethasone Regulates Macrophage and Cd4+Cd25+Cell Numbers in the Chicken Spleen

Full text
Author(s):
Calefi, A. S. [1] ; Quinteiro-Filho, W. M. [1] ; Fukushima, A. R. [2] ; da Cruz, D. S. G. [1] ; de Siqueira, A. [2] ; Salvagni, F. A. [2] ; Namazu, L. B. [1] ; Gomes, C. O. M. S. [1] ; Ferreira, A. J. P. [1] ; Palermo Neto, J. [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Neuroimmunomodulat Res Grp, Dept Pathol, Sch Vet Med & Anim Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Pathol, Sch Vet Med & Anim Sci, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Poultry Science; v. 18, n. 1, p. 93-99, JAN-MAR 2016.
Web of Science Citations: 4
Abstract

Dexamethasone (DEX) is a corticoid hormone that is experimentally used to mimic the effects of increased levels of endogenous corticosterone observed during the stress response. Currently, stress is considered one of the major predisposing factors for diseases in the poultry industry. The aim of this study was to analyze the effects of DEX and/or of a 20-fold coccidial vaccine dose on leukocyte phenotypes in the spleen and cecal tonsils of chickens. Twenty specific-pathogen-free (SPF) Leghorn chickens were divided into four groups: a non-treated group (NT), a DEX-treated group (Dex), a vaccinated group (V) and a DEX-treated+vaccinated group (Dex+V). On experimental day (ED) 42, each bird in the vaccinated groups received a anti-coccidial vaccine. DEX was injected in the birds of the Dex and Dex+V groups (0.9 mg/kg) onED42 and ED45. The immunophenotyping was performed by flow cytometry analysis of splenocytes and cecal tonsils cells onED48. DEX treatment per se was unable to change CD4(+)CD8(+), CD4(+)CD8(+) and CD4(-)CD8(+) populations with TCR gamma delta or CD28 in the spleen, or macrophages and T lymphocytes in the cecal tonsils. V group birds presented higher numbers of splenic macrophages compared with those measured in the Dex+V group. The number of CD4(+)CD25(+) cells in the spleen of birds of the V group was higher than those measured in the other experimental groups. Our data suggest that CD4(+)CD25(+) cells and macrophages might be influenced by DEX treatment in spleen, but not in the cecal tonsils of chickens inoculated with Eimeria. (AU)

FAPESP's process: 13/17408-2 - Effects of heat stress on immunity of broilers in an experimental model of avian necrotic enteritis
Grantee:Atilio Sersun Calefi
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/03103-2 - Evaluation of heat stress on the development of necrotic enteritis due to Clostridium perfringens in broilers
Grantee:Atilio Sersun Calefi
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 09/51886-3 - Neuroimmunomodulation: drugs, stress and cytokines on nervous, endocrine and immune systems relationships
Grantee:João Palermo Neto
Support Opportunities: Research Projects - Thematic Grants